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Journal of Bacteriology, December 2001, p. 6869-6874, Vol. 183, No. 23
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.23.6869-6874.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Noncoupled NADH:Ubiquinone Oxidoreductase of Azotobacter vinelandii Is Required for Diazotrophic Growth at High Oxygen Concentrations

Yulia V. Bertsova, Alexander V. Bogachev, and Vladimir P. Skulachev*

Department of Bioenergetics, A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia

Received 29 May 2001/Accepted 16 August 2001

The gene encoding the noncoupled NADH:ubiquinone oxidoreductase (NDH II) from Azotobacter vinelandii was cloned, sequenced, and used to construct an NDH II-deficient mutant strain. Compared to the wild type, this strain showed a marked decrease in respiratory activity. It was unable to grow diazotrophically at high aeration, while it was fully capable of growth at low aeration or in the presence of NH4+. This result suggests that the role of NDH II is as a vital component of the respiratory protection mechanism of the nitrogenase complex in A. vinelandii. It was also found that the oxidation of NADPH in the A. vinelandii respiratory chain is catalyzed solely by NDH II.

* Corresponding author. Mailing address: Department of Bioenergetics, A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia. Phone: (7) 095 939 55 30. Fax: (7) 095 939 03 38. E-mail: skulach{at}head.genebee.msu.su.

Journal of Bacteriology, December 2001, p. 6869-6874, Vol. 183, No. 23
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.23.6869-6874.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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