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Journal of Bacteriology, December 2001, p. 7007-7016, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7007-7016.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

H2O2-Forming NADH Oxidase with Diaphorase (Cytochrome) Activity from Archaeoglobus fulgidus

David W. Reed,dagger Jack Millstein, and Patricia L. Hartzell*

Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, Idaho 83844-3052

Received 15 March 2001/Accepted 21 September 2001

An enzyme exhibiting NADH oxidase (diaphorase) activity was isolated from the hyperthermophilic sulfate-reducing anaerobe Archaeoglobus fulgidus. N-terminal sequence of the protein indicates that it is coded for by open reading frame AF0395 in the A. fulgidus genome. The gene AF0395 was cloned and its product was purified from Escherichia coli. Like the native NADH oxidase (NoxA2), the recombinant NoxA2 (rNoxA2) has an apparent molecular mass of 47 kDa, requires flavin adenine dinucleotide for activity, has NADH-specific activity, and is thermostable. Hydrogen peroxide is the product of bivalent oxygen reduction by rNoxA2 with NADH. The rNoxA2 is an oxidase with diaphorase activity in the presence of electron acceptors such as tetrazolium and cytochrome c. During purification NoxA2 remains associated with the enzyme responsible for D-lactate oxidation, the D-lactate dehydrogenase (Dld), and the genes encoding NoxA2 and Dld are in the same transcription unit. Together these results suggest that NADH oxidase may be involved in electron transfer reactions resulting in sulfate respiration.

* Corresponding author. Mailing address: Department of Microbiology, University of Idaho, Moscow, ID 83844-3052. Phone: (208) 885-0572. Fax: (208) 885-6518. E-mail: hartzell{at}uidaho.edu.

dagger Present address: Idaho National Engineering and Environmental Laboratory, Idaho Falls, ID 83415.

Journal of Bacteriology, December 2001, p. 7007-7016, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7007-7016.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Case, C. L., Rodriguez, J. R., Mukhopadhyay, B. (2009). Characterization of an NADH oxidase of the flavin-dependent disulfide reductase family from Methanocaldococcus jannaschii. Microbiology 155: 69-79 [Abstract] [Full Text]  


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