This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kobayashi, K.
Right arrow Articles by Fujita, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kobayashi, K.
Right arrow Articles by Fujita, Y.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2001, p. 7365-7370, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7365-7370.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comprehensive DNA Microarray Analysis of Bacillus subtilis Two-Component Regulatory Systems

Kazuo Kobayashi,1 Mitsuo Ogura,2 Hirotake Yamaguchi,3 Ken-Ichi Yoshida,3 Naotake Ogasawara,1 Teruo Tanaka,2 and Yasutaro Fujita3,*

Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0101,1 Department of Marine Science and Technology, Tokai University, Shimizu, Shizuoka 424-8610,2 and Department of Biotechnology, Fukuyama University, Fukuyama, Hiroshima 729-0292,3 Japan

Received 2 July 2001/Accepted 25 August 2001

It has recently been shown through DNA microarray analysis of Bacillus subtilis two-component regulatory systems (DegS-DegU, ComP-ComA, and PhoR-PhoP) that overproduction of a response regulator of the two-component systems in the background of a deficiency of its cognate sensor kinase affects the regulation of genes, including its target ones. The genome-wide effect on gene expression caused by the overproduction was revealed by DNA microarray analysis. In the present work, we newly analyzed 24 two-component systems by means of this strategy, leaving out 8 systems to which it was unlikely to be applicable. This analysis revealed various target gene candidates for these two-component systems. It is especially notable that interesting interactions appeared to take place between several two-component systems. Moreover, the probable functions of some unknown two-component systems were deduced from the list of their target gene candidates. This work is heuristic but provides valuable information for further study toward a comprehensive understanding of the B. subtilis two-component regulatory systems. The DNA microarray data obtained in this work are available at the KEGG Expression Database website (http://www.genome.ad.jp/kegg/expression).

* Corresponding author. Mailing address: Department of Biotechnology, Faculty of Engineering, Fukuyama University, 985 Sanzo, Higashimura-cho, Fukuyama-shi, Hiroshima 729-0292, Japan. Phone: 81 849 36 2111. Fax: 81 849 36 2459. E-mail: yfujita{at}bt.fubt.fukuyama-u.ac.jp.

Journal of Bacteriology, December 2001, p. 7365-7370, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7365-7370.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Barbe, V., Cruveiller, S., Kunst, F., Lenoble, P., Meurice, G., Sekowska, A., Vallenet, D., Wang, T., Moszer, I., Medigue, C., Danchin, A. (2009). From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later. Microbiology 155: 1758-1775 [Abstract] [Full Text]  
  • Hussa, E. A., O'Shea, T. M., Darnell, C. L., Ruby, E. G., Visick, K. L. (2007). Two-Component Response Regulators of Vibrio fischeri: Identification, Mutagenesis, and Characterization. J. Bacteriol. 189: 5825-5838 [Abstract] [Full Text]  
  • Lulko, A. T., Veening, J.-W., Buist, G., Smits, W. K., Blom, E. J., Beekman, A. C., Bron, S., Kuipers, O. P. (2007). Production and Secretion Stress Caused by Overexpression of Heterologous {alpha}-Amylase Leads to Inhibition of Sporulation and a Prolonged Motile Phase in Bacillus subtilis. Appl. Environ. Microbiol. 73: 5354-5362 [Abstract] [Full Text]  
  • Hirooka, K., Kunikane, S., Matsuoka, H., Yoshida, K.-I., Kumamoto, K., Tojo, S., Fujita, Y. (2007). Dual Regulation of the Bacillus subtilis Regulon Comprising the lmrAB and yxaGH Operons and yxaF Gene by Two Transcriptional Repressors, LmrA and YxaF, in Response to Flavonoids. J. Bacteriol. 189: 5170-5182 [Abstract] [Full Text]  
  • Ogura, M., Tsukahara, K., Hayashi, K., Tanaka, T. (2007). The Bacillus subtilis NatK-NatR two-component system regulates expression of the natAB operon encoding an ABC transporter for sodium ion extrusion. Microbiology 153: 667-675 [Abstract] [Full Text]  
  • Matsuoka, H., Hirooka, K., Fujita, Y. (2007). Organization and Function of the YsiA Regulon of Bacillus subtilis Involved in Fatty Acid Degradation. J. Biol. Chem. 282: 5180-5194 [Abstract] [Full Text]  
  • Mascher, T., Helmann, J. D., Unden, G. (2006). Stimulus Perception in Bacterial Signal-Transducing Histidine Kinases. Microbiol. Mol. Biol. Rev. 70: 910-938 [Abstract] [Full Text]  
  • Jordan, S., Junker, A., Helmann, J. D., Mascher, T. (2006). Regulation of LiaRS-Dependent Gene Expression in Bacillus subtilis: Identification of Inhibitor Proteins, Regulator Binding Sites, and Target Genes of a Conserved Cell Envelope Stress-Sensing Two-Component System.. J. Bacteriol. 188: 5153-5166 [Abstract] [Full Text]  
  • Auchtung, J. M., Lee, C. A., Grossman, A. D. (2006). Modulation of the ComA-Dependent Quorum Response in Bacillus subtilis by Multiple Rap Proteins and Phr Peptides.. J. Bacteriol. 188: 5273-5285 [Abstract] [Full Text]  
  • Sitkiewicz, I., Musser, J. M. (2006). Expression Microarray and Mouse Virulence Analysis of Four Conserved Two-Component Gene Regulatory Systems in Group A Streptococcus. Infect. Immun. 74: 1339-1351 [Abstract] [Full Text]  
  • Sun, J., Zheng, L., Landwehr, C., Yang, J., Ji, Y. (2005). Identification of a Novel Essential Two-Component Signal Transduction System, YhcSR, in Staphylococcus aureus. J. Bacteriol. 187: 7876-7880 [Abstract] [Full Text]  
  • Azcarate-Peril, M. A., McAuliffe, O., Altermann, E., Lick, S., Russell, W. M., Klaenhammer, T. R. (2005). Microarray Analysis of a Two-Component Regulatory System Involved in Acid Resistance and Proteolytic Activity in Lactobacillus acidophilus. Appl. Environ. Microbiol. 71: 5794-5804 [Abstract] [Full Text]  
  • Satomura, T., Shimura, D., Asai, K., Sadaie, Y., Hirooka, K., Fujita, Y. (2005). Enhancement of Glutamine Utilization in Bacillus subtilis through the GlnK-GlnL Two-Component Regulatory System. J. Bacteriol. 187: 4813-4821 [Abstract] [Full Text]  
  • Vemuri, G. N., Aristidou, A. A. (2005). Metabolic Engineering in the -omics Era: Elucidating and Modulating Regulatory Networks. Microbiol. Mol. Biol. Rev. 69: 197-216 [Abstract] [Full Text]  
  • Serizawa, M., Sekiguchi, J. (2005). The Bacillus subtilis YdfHI two-component system regulates the transcription of ydfJ, a member of the RND superfamily. Microbiology 151: 1769-1778 [Abstract] [Full Text]  
  • Vetter, S. M., Schlievert, P. M. (2005). Glycerol Monolaurate Inhibits Virulence Factor Production in Bacillus anthracis. Antimicrob. Agents Chemother. 49: 1302-1305 [Abstract] [Full Text]  
  • Kuchma, S. L., Connolly, J. P., O'Toole, G. A. (2005). A Three-Component Regulatory System Regulates Biofilm Maturation and Type III Secretion in Pseudomonas aeruginosa. J. Bacteriol. 187: 1441-1454 [Abstract] [Full Text]  
  • Hartig, E., Geng, H., Hartmann, A., Hubacek, A., Munch, R., Ye, R. W., Jahn, D., Nakano, M. M. (2004). Bacillus subtilis ResD Induces Expression of the Potential Regulatory Genes yclJK upon Oxygen Limitation. J. Bacteriol. 186: 6477-6484 [Abstract] [Full Text]  
  • Ng, W.-L., Winkler, M. E. (2004). Singular structures and operon organizations of essential two-component systems in species of Streptococcus. Microbiology 150: 3096-3098 [Full Text]  
  • Mascher, T., Zimmer, S. L., Smith, T.-A., Helmann, J. D. (2004). Antibiotic-Inducible Promoter Regulated by the Cell Envelope Stress-Sensing Two-Component System LiaRS of Bacillus subtilis. Antimicrob. Agents Chemother. 48: 2888-2896 [Abstract] [Full Text]  
  • Joseph, P., Guiseppi, A., Sorokin, A., Denizot, F. (2004). Characterization of the Bacillus subtilis YxdJ response regulator as the inducer of expression for the cognate ABC transporter YxdLM. Microbiology 150: 2609-2617 [Abstract] [Full Text]  
  • Liu, J., Tan, K., Stormo, G. D. (2003). Computational identification of the Spo0A-phosphate regulon that is essential for the cellular differentiation and development in Gram-positive spore-forming bacteria. Nucleic Acids Res 31: 6891-6903 [Abstract] [Full Text]  
  • Tojo, S., Matsunaga, M., Matsumoto, T., Kang, C.-M., Yamaguchi, H., Asai, K., Sadaie, Y., Yoshida, K.-i., Fujita, Y. (2003). Organization and Expression of the Bacillus subtilis sigY Operon. J Biochem 134: 935-946 [Abstract] [Full Text]  
  • Steil, L., Hoffmann, T., Budde, I., Volker, U., Bremer, E. (2003). Genome-Wide Transcriptional Profiling Analysis of Adaptation of Bacillus subtilis to High Salinity. J. Bacteriol. 185: 6358-6370 [Abstract] [Full Text]  
  • Tanaka, K., Kobayashi, K., Ogasawara, N. (2003). The Bacillus subtilis YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium. Microbiology 149: 2317-2329 [Abstract] [Full Text]  
  • Doan, T., Servant, P., Tojo, S., Yamaguchi, H., Lerondel, G., Yoshida, K.-I., Fujita, Y., Aymerich, S. (2003). The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate. Microbiology 149: 2331-2343 [Abstract] [Full Text]  
  • Molle, V., Nakaura, Y., Shivers, R. P., Yamaguchi, H., Losick, R., Fujita, Y., Sonenshein, A. L. (2003). Additional Targets of the Bacillus subtilis Global Regulator CodY Identified by Chromatin Immunoprecipitation and Genome-Wide Transcript Analysis. J. Bacteriol. 185: 1911-1922 [Abstract] [Full Text]  
  • Stanley, N. R., Britton, R. A., Grossman, A. D., Lazazzera, B. A. (2003). Identification of Catabolite Repression as a Physiological Regulator of Biofilm Formation by Bacillus subtilis by Use of DNA Microarrays. J. Bacteriol. 185: 1951-1957 [Abstract] [Full Text]  
  • Chen, Y., Birck, C., Samama, J.-P., Hulett, F. M. (2003). Residue R113 Is Essential for PhoP Dimerization and Function: a Residue Buried in the Asymmetric PhoP Dimer Interface Determined in the PhoPN Three-Dimensional Crystal Structure. J. Bacteriol. 185: 262-273 [Abstract] [Full Text]  
  • Masuda, N., Church, G. M. (2002). Escherichia coli Gene Expression Responsive to Levels of the Response Regulator EvgA. J. Bacteriol. 184: 6225-6234 [Abstract] [Full Text]  
  • Beckering, C. L., Steil, L., Weber, M. H. W., Volker, U., Marahiel, M. A. (2002). Genomewide Transcriptional Analysis of the Cold Shock Response in Bacillus subtilis. J. Bacteriol. 184: 6395-6402 [Abstract] [Full Text]  
  • Hancock, L., Perego, M. (2002). Two-Component Signal Transduction in Enterococcus faecalis. J. Bacteriol. 184: 5819-5825 [Full Text]  
  • Yamaguchi, K., Suzuki, I., Yamamoto, H., Lyukevich, A., Bodrova, I., Los, D. A., Piven, I., Zinchenko, V., Kanehisa, M., Murata, N. (2002). A Two-Component Mn2+-Sensing System Negatively Regulates Expression of the mntCAB Operon in Synechocystis. Plant Cell 14: 2901-2913 [Abstract] [Full Text]  
  • Ogura, M., Yamaguchi, H., Kobayashi, K., Ogasawara, N., Fujita, Y., Tanaka, T. (2002). Whole-Genome Analysis of Genes Regulated by the Bacillus subtilis Competence Transcription Factor ComK. J. Bacteriol. 184: 2344-2351 [Abstract] [Full Text]  
  • Perego, M., Hoch, J. A. (2001). Functional Genomics of Gram-Positive Microorganisms: Review of the Meeting, San Diego, California, 24 to 28 June 2001. J. Bacteriol. 183: 6973-6978 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»