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Journal of Bacteriology, December 2001, p. 7403-7407, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7403-7407.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The lytB Gene of Escherichia coli Is Essential and Specifies a Product Needed for Isoprenoid Biosynthesis

Sean McAteer, Andrew Coulson, Neil McLennan,dagger and Millicent Masters*

Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland

Received 21 May 2001/Accepted 12 September 2001

LytB and GcpE, because they are codistributed with other pathway enzymes, have been predicted to catalyze unknown steps in the nonmevalonate pathway for isoprenoid biosynthesis. We constructed a conditional Escherichia coli lytB mutant and found that LytB is essential for survival and that depletion of LytB results in cell lysis, which is consistent with a role for this protein in isoprenoid biosynthesis. Alcohols which can be converted to pathway intermediates beyond the hypothesized LytB step(s) support limited growth of E. coli lytB mutants. An informatic analysis of protein structure suggested that GcpE is a globular protein of the TIM barrel class and that LytB is also a globular protein. Possible biochemical roles for LytB and GcpE are suggested.

* Corresponding author. Mailing address: Institute of Cell and Molecular Biology, University of Edinburgh, Kings' Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland. Phone: 44 131 650 5355. Fax: 44 131 650 8650. E-mail: M.Masters{at}ed.ac.uk.

dagger Present address: CJD Surveillance Unit, Department of Pathology, University of Edinburgh, Western General Hospital, Edinburgh, Scotland.

Journal of Bacteriology, December 2001, p. 7403-7407, Vol. 183, No. 24
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.24.7403-7407.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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