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Journal of Bacteriology, February 2001, p. 1096-1100, Vol. 183, No. 3
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.3.1096-1100.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Transcript Analysis of Multiple Copies of amo (Encoding Ammonia Monooxygenase) and hao (Encoding Hydroxylamine Oxidoreductase) in Nitrosomonas europaea

Norman G. Hommes, Luis A. Sayavedra-Soto, and Daniel J. Arp*

Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331-2902

Received 3 August 2000/Accepted 26 October 2000

The genes encoding ammonia monooxygenase (amoCAB), hydroxylamine oxidoreductase (hao), and the c-type cytochrome c-554 (hcy) are present in multiple copies in the genome of Nitrosomonas europaea. The upstream regions of the two copies of amoC, the three copies of hao, and one copy of hcy were cloned and sequenced. Primer extension reactions were done to identify transcription start sites for these genes, as well as for amoA. Putative sigma 70 promoter sequences were found associated with all but one of the mapped transcription start sites. Primer extensions were done with amoC primers using RNA harvested from cells incubated with and without ammonium. The experiments suggested that N. europaea cells may be able to use different promoters in the presence and absence of ammonium.

* Corresponding author. Mailing address: Department of Botany and Plant Pathology. Oregon State University, 2082 Cordley, Corvallis, OR 97331-2902. Phone: (541) 737-4214. Fax: (541) 737-3573. E-mail: arpd{at}bcc.orst.edu.

Journal of Bacteriology, February 2001, p. 1096-1100, Vol. 183, No. 3
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.3.1096-1100.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Berube, P. M., Samudrala, R., Stahl, D. A. (2007). Transcription of All amoC Copies Is Associated with Recovery of Nitrosomonas europaea from Ammonia Starvation. J. Bacteriol. 189: 3935-3944 [Abstract] [Full Text]  
  • Bergmann, D. J., Hooper, A. B., Klotz, M. G. (2005). Structure and Sequence Conservation of hao Cluster Genes of Autotrophic Ammonia-Oxidizing Bacteria: Evidence for Their Evolutionary History. Appl. Environ. Microbiol. 71: 5371-5382 [Abstract] [Full Text]  
  • Wei, X., Sayavedra-Soto, L. A., Arp, D. J. (2004). The transcription of the cbb operon in Nitrosomonas europaea. Microbiology 150: 1869-1879 [Abstract] [Full Text]  


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