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Journal of Bacteriology, February 2001, p. 1101-1105, Vol. 183, No. 3
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.3.1101-1105.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Experimental Evolution of Enzyme Temperature Activity Profile: Selection In Vivo and Characterization of Low-Temperature-Adapted Mutants of Pyrococcus furiosus Ornithine Carbamoyltransferase

Martine Roovers,1 Rony Sanchez,1 Christianne Legrain,2 and Nicolas Glansdorff1,2,3,*

Department of Microbiology, Flanders Interuniversity Institute for Biotechnology (VIB),1 Department of Microbiology, Free University of Brussels (VUB),3 and Research Institute J. M. Wiame,2 B-1070 Brussels, Belgium

Received 26 June 2000/Accepted 25 October 2000

We have obtained mutants of Pyrococcus furiosus ornithine carbamoyltransferase active at low temperatures by selecting for complementation of an appropriate yeast mutant after in vivo mutagenesis. The mutants were double ones, still complementing at 15°C, a temperature already in the psychrophilic range. Their kinetic analysis is reported.

* Corresponding author. Mailing address: Flanders Interuniversity Institute for Biotechnology (VIB), Free University of Brussels (VUB), and Research Institute J. M. Wiame, E. Grysonlaan 1, B-1070 Brussels, Belgium. Phone: 32 2 5267275. Fax: 32 2 5267273. E-mail: ceriair{at}ulb.ac.be.

Journal of Bacteriology, February 2001, p. 1101-1105, Vol. 183, No. 3
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.3.1101-1105.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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