This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by O'Connor, K. E.
Right arrow Articles by Duetz, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by O'Connor, K. E.
Right arrow Articles by Duetz, W.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2001, p. 928-933, Vol. 183, No. 3
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.3.928-933.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

p-Hydroxyphenylacetic Acid Metabolism in Pseudomonas putida F6

Kevin E. O'Connor,* Bernard Witholt, and Wouter Duetz

Institut fur Biotechnologie, ETH Honggerberg, Zurich, Switzerland

Received 7 August 2000/Accepted 7 November 2000

Pseudomonas putida F6 was found to metabolize p-hydroxyphenylacetic acid through 3,4-dihydroxyphenylacetic acid, 3,4-dihydroxymandelic acid, and 3,4-dihydroxybenzaldehyde. Cell extracts of P. putida F6 catalyze the NAD(P)H-independent hydroxylation of p-hydroxyphenylacetic acid to 3,4-dihydroxyphenylacetic acid which is further oxidized to 3,4-dihydroxymandelic acid. Oxidation and decarboxylation of the latter yields 3,4-dihydroxybenzaldehyde. A red-brown color accompanies all of the above enzyme activities and is probably due to the polymerization of quinone-like compounds. 3,4-Dihydroxybenzaldehyde is further metabolized through extradiol ring cleavage.

* Corresponding author. Present address: Department of Industrial Microbiology, Ardmore House, National University of Ireland, Belfield, Dublin 4, Ireland. Phone: 353 1 706 1307. Fax: 353 1 706 1183. E-mail: kevin.oconnor{at}ucd.ie.

Journal of Bacteriology, February 2001, p. 928-933, Vol. 183, No. 3
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.3.928-933.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nikodinovic-Runic, J., Flanagan, M., Hume, A. R., Cagney, G., O'Connor, K. E. (2009). Analysis of the Pseudomonas putida CA-3 proteome during growth on styrene under nitrogen-limiting and non-limiting conditions. Microbiology 155: 3348-3361 [Abstract] [Full Text]  
  • Arias-Barrau, E., Sandoval, A., Naharro, G., Olivera, E. R., Luengo, J. M. (2005). A Two-component Hydroxylase Involved in the Assimilation of 3-Hydroxyphenyl Acetate in Pseudomonas putida. J. Biol. Chem. 280: 26435-26447 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»