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Journal of Bacteriology, February 2001, p. 1225-1232, Vol. 183, No. 4
School of Biological Sciences, University of
Wales Bangor, Bangor, Gwynedd LL57 2UW, Wales, United Kingdom
Received 9 August 2000/Accepted 16 November 2000
Pseudomonas putida strain TW3 is able to metabolize
4-nitrotoluene via 4-nitrobenzoate (4NBen) and 3, 4-dihydroxybenzoic
acid (protocatechuate [PCA]) to central metabolites. We have cloned, sequenced, and characterized a 6-kbp fragment of TW3 DNA which contains
five genes, two of which encode the enzymes involved in the
catabolism of 4NBen to PCA. In order, they encode a 4NBen reductase
(PnbA) which is responsible for catalyzing the direct reduction of
4NBen to 4-hydroxylaminobenzoate with the oxidation of 2 mol of NADH
per mol of 4NBen, a reductase-like enzyme (Orf1) which appears to have
no function in the pathway, a regulator protein (PnbR) of the LysR
family, a 4-hydroxylaminobenzoate lyase (PnbB) which catalyzes the
conversion of 4-hydroxylaminobenzoate to PCA and ammonium, and a second
lyase-like enzyme (Orf2) which is closely associated with
pnbB but appears to have no function in the pathway. The
central pnbR gene is transcribed in the opposite direction
to the other four genes. These genes complete the characterization of
the whole pathway of 4-nitrotoluene catabolism to the ring cleavage
substrate PCA in P. putida strain TW3.
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.4.1225-1232.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Cloning and Characterization of the pnb Genes,
Encoding Enzymes for 4-Nitrobenzoate Catabolism in
Pseudomonas putida TW3
*
Corresponding author. Mailing address: School of
Biological Sciences, University of Wales Bangor, Bangor, Gwynedd LL57
2UW, Wales, United Kingdom. Phone: (44) 1248 382363. Fax: (44) 1248 370731. E-mail: P.A.Williams{at}bangor.ac.uk
Journal of Bacteriology, February 2001, p. 1225-1232, Vol. 183, No. 4
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.4.1225-1232.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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