This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by O'Donnell, S. M.
Right arrow Articles by Janssen, G. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by O'Donnell, S. M.
Right arrow Articles by Janssen, G. R.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2001, p. 1277-1283, Vol. 183, No. 4
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.4.1277-1283.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The Initiation Codon Affects Ribosome Binding and Translational Efficiency in Escherichia coli of cI mRNA with or without the 5' Untranslated Leader

Sean M. O'Donnell and Gary R. Janssen*

Department of Microbiology, Miami University, Oxford, Ohio 45056

Received 5 July 2000/Accepted 17 November 2000

Translational efficiency of an AUG, CUG, GUG, or UUG initiation codon was measured for the naturally leaderless cI mRNA from bacteriophage lambda . In a cI-lacZ translational fusion, only AUG supported a high level of expression; GUG supported a low level of expression, while UUG and CUG expression was barely above background levels. Addition of an untranslated lac leader and Shine-Dalgarno sequence to cI increased expression but still showed a dependence on an AUG for maximum expression. cI-lacZ mRNA with an AUG initiation codon showed a greater in vitro ribosome binding strength and a higher level of full-length in vivo mRNA, suggesting that the initiation codon is an important determinant of ribosome binding strength and translational efficiency for mRNA with or without the 5' untranslated leader.

* Corresponding author. Mailing address: Department of Microbiology, Miami University, Oxford, OH 45056. Phone: (513) 529-1694. Fax: (513) 529-2431. E-mail: janssegr{at}muohio.edu

Journal of Bacteriology, February 2001, p. 1277-1283, Vol. 183, No. 4
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.4.1277-1283.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Su'etsugu, M., Nakamura, K., Keyamura, K., Kudo, Y., Katayama, T. (2008). Hda Monomerization by ADP Binding Promotes Replicase Clamp-mediated DnaA-ATP Hydrolysis. J. Biol. Chem. 283: 36118-36131 [Abstract] [Full Text]  
  • Brock, J. E., Pourshahian, S., Giliberti, J., Limbach, P. A., Janssen, G. R. (2008). Ribosomes bind leaderless mRNA in Escherichia coli through recognition of their 5'-terminal AUG. RNA 14: 2159-2169 [Abstract] [Full Text]  
  • Brock, J. E., Paz, R. L., Cottle, P., Janssen, G. R. (2007). Naturally Occurring Adenines within mRNA Coding Sequences Affect Ribosome Binding and Expression in Escherichia coli. J. Bacteriol. 189: 501-510 [Abstract] [Full Text]  
  • Marszalkowski, M., Teune, J.-H., Steger, G., Hartmann, R. K., Willkomm, D. K. (2006). Thermostable RNase P RNAs lacking P18 identified in the Aquificales. RNA 12: 1915-1921 [Abstract] [Full Text]  
  • Day, J. M., Janssen, G. R. (2004). Isolation and Characterization of Ribosomes and Translation Initiation Factors from the Gram-Positive Soil Bacterium Streptomyces lividans. J. Bacteriol. 186: 6864-6875 [Abstract] [Full Text]  
  • Miller, E. S., Kutter, E., Mosig, G., Arisaka, F., Kunisawa, T., Ruger, W. (2003). Bacteriophage T4 Genome. Microbiol. Mol. Biol. Rev. 67: 86-156 [Abstract] [Full Text]  
  • O'Donnell, S. M., Janssen, G. R. (2002). Leaderless mRNAs Bind 70S Ribosomes More Strongly than 30S Ribosomal Subunits in Escherichia coli. J. Bacteriol. 184: 6730-6733 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»