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Journal of Bacteriology, February 2001, p. 1466-1471, Vol. 183, No. 4
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.4.1466-1471.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Drosophila as a Model Host for Pseudomonas aeruginosa Infection

David A. D'Argenio, Larry A. Gallagher, Celeste A. Berg, and Colin Manoil*

Department of Genetics, University of Washington, Seattle, Washington 98195-7360

Received 7 September 2000/Accepted 16 November 2000

Using the fruit fly Drosophila melanogaster as model host, we have identified mutants of the bacterium Pseudomonas aeruginosa with reduced virulence. Strikingly, all strains strongly impaired in fly killing also lacked twitching motility; most such strains had a mutation in pilGHIJKL chpABCDE, a gene cluster known to be required for twitching motility and potentially encoding a signal transduction system. The pil chp genes appear to control the expression of additional virulence factors, however, since the wild-type fly-killing phenotype of a subset of mutants isolated on the basis of their compact colony morphology indicated that twitching motility itself was not required for full virulence in the fly.

* Corresponding author, Mailing address: Department of Genetics, University of Washington, 1959 NE Pacific St.---J205, Box 357360, Seattle, WA 98195-7360. Phone: (206)-543-7800. Fax: (206) 543-0754. E-mail: manoil{at}u.washington.edu.

Journal of Bacteriology, February 2001, p. 1466-1471, Vol. 183, No. 4
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.4.1466-1471.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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