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Journal of Bacteriology, February 2001, p. 1482-1488, Vol. 183, No. 4
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.4.1482-1488.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification and Characterization of gsp65, an Organic Hydroperoxide Resistance (ohr) Gene Encoding a General Stress Protein in Enterococcus faecalis

Alain Rincé,* Jean-Christophe Giard, Vianney Pichereau, Sigrid Flahaut,dagger and Yanick Auffray

Laboratoire de Microbiologie de l'Environnement, IRBA, Université de Caen, 14032 Caen Cedex, France

Received 28 August 2000/Accepted 1 November 2000

The Enterococcus faecalis general stress protein Gsp65 has been purified from two-dimensional gel electrophoresis. Determination of its N-terminal sequence and characterization of the corresponding gene revealed that the gsp65 product is a 133-amino-acid protein sharing homologies with organic hydroperoxide resistance (Ohr) proteins. Transcriptional analysis of gsp65 gave evidence for a monocistronic mRNA initiated 52 nucleotides upstream of the ATG start codon and for an induction in response to hydrogen peroxide, heat shock, acid pH, detergents, ethanol, sodium chloride, and tert-butylhydroperoxide (tBOOH). A gsp65 mutant showed increased sensitivity to the organic hydroperoxide tBOOH and to ethanol.

* Corresponding author. Mailing address: Laboratoire de Microbiologie de l'Environnement, IRBA, Université de Caen, 14032 Caen Cedex, France. Phone: 00-33-2-31-56-55-23. Fax 00-33-2-31-56-53-11. E.mail: rince{at}ibba.unicaen.fr.

dagger Present address: Laboratoire de Biologie Cellulaire et Moléculaire, Université du Littoral-Côte d'Opale, 62327 Boulogne sur Mer Cedex, France.

Journal of Bacteriology, February 2001, p. 1482-1488, Vol. 183, No. 4
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.4.1482-1488.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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