This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Laue, H.
Right arrow Articles by Cook, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Laue, H.
Right arrow Articles by Cook, A. M.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2001, p. 1727-1733, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1727-1733.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Dissimilatory Sulfite Reductase (Desulfoviridin) of the Taurine-Degrading, Non-Sulfate-Reducing Bacterium Bilophila wadsworthia RZATAU Contains a Fused DsrB-DsrD Subunit

Heike Laue,1,* Michael Friedrich,2 Jürgen Ruff,1 and Alasdair M. Cook1

Fachbereich Biologie, Universität Konstanz, D-78457 Konstanz,1 and Max-Planck-Institut für Terrestrische Mikrobiologie, D-35043 Marburg,2 Germany

Received 16 June 2000/Accepted 6 December 2000

A dissimilatory sulfite reductase (DSR) was purified from the anaerobic, taurine-degrading bacterium Bilophila wadsworthia RZATAU to apparent homogeneity. The enzyme is involved in energy conservation by reducing sulfite, which is formed during the degradation of taurine as an electron acceptor, to sulfide. According to its UV-visible absorption spectrum with maxima at 392, 410, 583, and 630 nm, the enzyme belongs to the desulfoviridin type of DSRs. The sulfite reductase was isolated as an alpha 2beta 2gamma n (n >=  2) multimer with a native size of 285 kDa as determined by gel filtration. We have sequenced the genes encoding the alpha  and beta  subunits (dsrA and dsrB, respectively), which probably constitute one operon. dsrA and dsrB encode polypeptides of 49 (alpha ) and 54 kDa (beta ) which show significant similarities to the homologous subunits of other DSRs. The dsrB gene product of B. wadsworthia is apparently a fusion protein of dsrB and dsrD. This indicates a possible functional role of DsrD in DSR function because of its presence as a fusion protein as an integral part of the DSR holoenzyme in B. wadsworthia. A phylogenetic analysis using the available Dsr sequences revealed that B. wadsworthia grouped with its closest 16S rDNA relative Desulfovibrio desulfuricans Essex 6.

* Corresponding author. Mailing address: Fachbereich Biologie, Universität Konstanz, Universitätsstr. 10, D-78457 Konstanz, Germany. Phone: 7531 88 4385. Fax: 7531 88 29 66. E-mail: heike_laue{at}hotmail.com.

Journal of Bacteriology, March 2001, p. 1727-1733, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1727-1733.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ridlon, J. M., Kang, D.-J., Hylemon, P. B. (2006). Bile salt biotransformations by human intestinal bacteria. J. Lipid Res. 47: 241-259 [Abstract] [Full Text]  
  • Mussmann, M., Richter, M., Lombardot, T., Meyerdierks, A., Kuever, J., Kube, M., Glockner, F. O., Amann, R. (2005). Clustered Genes Related to Sulfate Respiration in Uncultured Prokaryotes Support the Theory of Their Concomitant Horizontal Transfer. J. Bacteriol. 187: 7126-7137 [Abstract] [Full Text]  
  • Warren, Y. A., Citron, D. M., Merriam, C. V., Goldstein, E. J. C. (2005). Biochemical Differentiation and Comparison of Desulfovibrio Species and Other Phenotypically Similar Genera. J. Clin. Microbiol. 43: 4041-4045 [Abstract] [Full Text]  
  • Zverlov, V., Klein, M., Lucker, S., Friedrich, M. W., Kellermann, J., Stahl, D. A., Loy, A., Wagner, M. (2005). Lateral Gene Transfer of Dissimilatory (Bi)Sulfite Reductase Revisited. J. Bacteriol. 187: 2203-2208 [Abstract] [Full Text]  
  • Dahl, C., Engels, S., Pott-Sperling, A. S., Schulte, A., Sander, J., Lubbe, Y., Deuster, O., Brune, D. C. (2005). Novel Genes of the dsr Gene Cluster and Evidence for Close Interaction of Dsr Proteins during Sulfur Oxidation in the Phototrophic Sulfur Bacterium Allochromatium vinosum. J. Bacteriol. 187: 1392-1404 [Abstract] [Full Text]  
  • Friedrich, M. W. (2002). Phylogenetic Analysis Reveals Multiple Lateral Transfers of Adenosine-5'-Phosphosulfate Reductase Genes among Sulfate-Reducing Microorganisms. J. Bacteriol. 184: 278-289 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»