This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jack, R. L.
Right arrow Articles by Palmer, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jack, R. L.
Right arrow Articles by Palmer, T.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2001, p. 1801-1804, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1801-1804.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Constitutive Expression of Escherichia coli tat Genes Indicates an Important Role for the Twin-Arginine Translocase during Aerobic and Anaerobic Growth

Rachael L. Jack,1,2 Frank Sargent,1,2 Ben C. Berks,1 Gary Sawers,2 and Tracy Palmer1,2,*

Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ,1 and Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH,2 United Kingdom

Received 7 September 2000/Accepted 30 November 2000

The transcription start sites for the tatABCD and tatE loci, encoding components of the Tat (twin-arginine translocase) protein export pathway, have been identified. Expression studies indicate that the tatABCD and tatE transcription units are expressed constitutively. Translational fusion experiments suggest that TatA is synthesized at a much higher level than the other Tat proteins.

* Corresponding author. Mailing address: Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, United Kingdom. Phone: 44 (0)1603 450726. Fax: 44 (0)1603 450018. E-mail: tracy.palmer{at}bbsrc.ac.uk.

Journal of Bacteriology, March 2001, p. 1801-1804, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1801-1804.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Berthelmann, F., Mehner, D., Richter, S., Lindenstrauss, U., Lunsdorf, H., Hause, G., Bruser, T. (2008). Recombinant Expression of tatABC and tatAC Results in the Formation of Interacting Cytoplasmic TatA Tubes in Escherichia coli. J. Biol. Chem. 283: 25281-25289 [Abstract] [Full Text]  
  • Donald, J. W., Hicks, M. G., Richardson, D. J., Palmer, T. (2008). The c-Type Cytochrome OmcA Localizes to the Outer Membrane upon Heterologous Expression in Escherichia coli. J. Bacteriol. 190: 5127-5131 [Abstract] [Full Text]  
  • Barrett, C. M. L., Freudl, R., Robinson, C. (2007). Twin Arginine Translocation (Tat)-dependent Export in the Apparent Absence of TatABC or TatA Complexes Using Modified Escherichia coli TatA Subunits That Substitute for TatB. J. Biol. Chem. 282: 36206-36213 [Abstract] [Full Text]  
  • Punginelli, C., Maldonado, B., Grahl, S., Jack, R., Alami, M., Schroder, J., Berks, B. C., Palmer, T. (2007). Cysteine Scanning Mutagenesis and Topological Mapping of the Escherichia coli Twin-Arginine Translocase TatC Component. J. Bacteriol. 189: 5482-5494 [Abstract] [Full Text]  
  • Kreutzenbeck, P., Kroger, C., Lausberg, F., Blaudeck, N., Sprenger, G. A., Freudl, R. (2007). Escherichia coli Twin Arginine (Tat) Mutant Translocases Possessing Relaxed Signal Peptide Recognition Specificities. J. Biol. Chem. 282: 7903-7911 [Abstract] [Full Text]  
  • Lee, P. A., Orriss, G. L., Buchanan, G., Greene, N. P., Bond, P. J., Punginelli, C., Jack, R. L., Sansom, M. S. P., Berks, B. C., Palmer, T. (2006). Cysteine-scanning Mutagenesis and Disulfide Mapping Studies of the Conserved Domain of the Twin-arginine Translocase TatB Component. J. Biol. Chem. 281: 34072-34085 [Abstract] [Full Text]  
  • Kikuchi, Y., Date, M., Itaya, H., Matsui, K., Wu, L.-F. (2006). Functional Analysis of the Twin-Arginine Translocation Pathway in Corynebacterium glutamicum ATCC 13869. Appl. Environ. Microbiol. 72: 7183-7192 [Abstract] [Full Text]  
  • Schreiber, S., Stengel, R., Westermann, M., Volkmer-Engert, R., Pop, O. I., Muller, J. P. (2006). Affinity of TatCd for TatAd Elucidates Its Receptor Function in the Bacillus subtilis Twin Arginine Translocation (Tat) Translocase System. J. Biol. Chem. 281: 19977-19984 [Abstract] [Full Text]  
  • Dibden, D. P., Green, J. (2005). In vivo cycling of the Escherichia coli transcription factor FNR between active and inactive states. Microbiology 151: 4063-4070 [Abstract] [Full Text]  
  • Ray, N., Nenninger, A., Mullineaux, C. W., Robinson, C. (2005). Location and Mobility of Twin Arginine Translocase Subunits in the Escherichia coli Plasma Membrane. J. Biol. Chem. 280: 17961-17968 [Abstract] [Full Text]  
  • Hicks, M. G., Lee, P. A., Georgiou, G., Berks, B. C., Palmer, T. (2005). Positive Selection for Loss-of-Function tat Mutations Identifies Critical Residues Required for TatA Activity. J. Bacteriol. 187: 2920-2925 [Abstract] [Full Text]  
  • Blaudeck, N., Kreutzenbeck, P., Muller, M., Sprenger, G. A., Freudl, R. (2005). Isolation and Characterization of Bifunctional Escherichia coli TatA Mutant Proteins That Allow Efficient Tat-dependent Protein Translocation in the Absence of TatB. J. Biol. Chem. 280: 3426-3432 [Abstract] [Full Text]  
  • Ize, B., Porcelli, I., Lucchini, S., Hinton, J. C., Berks, B. C., Palmer, T. (2004). Novel Phenotypes of Escherichia coli tat Mutants Revealed by Global Gene Expression and Phenotypic Analysis. J. Biol. Chem. 279: 47543-47554 [Abstract] [Full Text]  
  • Gouffi, K., Gerard, F., Santini, C.-L., Wu, L.-F. (2004). Dual Topology of the Escherichia coli TatA Protein. J. Biol. Chem. 279: 11608-11615 [Abstract] [Full Text]  
  • Jones, S. E., Lloyd, L. J., Tan, K. K., Buck, M. (2003). Secretion Defects That Activate the Phage Shock Response of Escherichia coli. J. Bacteriol. 185: 6707-6711 [Abstract] [Full Text]  
  • Papish, A. L., Ladner, C. L., Turner, R. J. (2003). The Twin-arginine Leader-binding Protein, DmsD, Interacts with the TatB and TatC Subunits of the Escherichia coli Twin-arginine Translocase. J. Biol. Chem. 278: 32501-32506 [Abstract] [Full Text]  
  • Palmer, T., Berks, B. C. (2003). Moving folded proteins across the bacterial cell membrane. Microbiology 149: 547-556 [Abstract] [Full Text]  
  • Lee, P. A., Buchanan, G., Stanley, N. R., Berks, B. C., Palmer, T. (2002). Truncation Analysis of TatA and TatB Defines the Minimal Functional Units Required for Protein Translocation. J. Bacteriol. 184: 5871-5879 [Abstract] [Full Text]  
  • Pop, O., Martin, U., Abel, C., Muller, J. P. (2002). The Twin-arginine Signal Peptide of PhoD and the TatAd/Cd Proteins of Bacillus subtilis Form an Autonomous Tat Translocation System. J. Biol. Chem. 277: 3268-3273 [Abstract] [Full Text]  
  • Bolhuis, A., Mathers, J. E., Thomas, J. D., Barrett, C. M. L., Robinson, C. (2001). TatB and TatC Form a Functional and Structural Unit of the Twin-arginine Translocase from Escherichia coli. J. Biol. Chem. 276: 20213-20219 [Abstract] [Full Text]  
  • Scott, C., Green, J. (2002). Miscoordination of the Iron-Sulfur Clusters of the Anaerobic Transcription Factor, FNR, Allows Simple Repression but Not Activation. J. Biol. Chem. 277: 1749-1754 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»