This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Doree, S. M.
Right arrow Articles by Mulks, M. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Doree, S. M.
Right arrow Articles by Mulks, M. H.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2001, p. 1983-1989, Vol. 183, No. 6
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.6.1983-1989.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of an Actinobacillus pleuropneumoniae Consensus Promoter Structure

Scott M. Doree and Martha H. Mulks*

Department of Microbiology, Michigan State University, East Lansing, Michigan 48824-1101

Received 15 November 2000/Accepted 20 December 2000

Actinobacillus pleuropneumoniae promoter-containing clones were isolated from a genomic DNA library constructed in our lVET promoter trap vector pTF86. The promoter-containing clones were identified by their ability to drive expression of the promoterless luxAB genes of Vibrio harveyi. The degree of expression was quantifiable, and only high-expression or "hot" promoters were used for this study. Nine clones were sequenced, and their transcriptional start sites were determined by primer extension. The sequences upstream of the start site were aligned, and a consensus promoter structure for A. pleuropneumoniae was identified. The consensus promoter sequence for A. pleuropneumoniae was found to be TATAAT and TTG/AAA, centered approximately 10 and 35 bp upstream of the transcriptional start site, respectively. A comparison of the A. pleuropneumoniae consensus with other prokaryotic consensus promoters showed that the A. pleuropneumoniae consensus promoter is similar to that found in other eubacteria in terms of sequence, with an identical -10 element and a similar but truncated -35 element. However, the A. pleuropneumoniae consensus promoter is unique in the spacing between the -10 and -35 elements. The promoter spacing was analyzed by site-directed mutagenesis, which demonstrated that optimal spacing for an A. pleuropneumoniae promoter is shorter than the spacing identified for Escherichia coli and Bacillus subtilis promoters.

* Corresponding author. Mailing address: Department of Microbiology, Michigan State University, East Lansing, MI 48824-1101. Phone: (517) 355-6515. Fax: (517) 353-8957. E-mail: mulks{at}pilot.msu.edu.

Journal of Bacteriology, March 2001, p. 1983-1989, Vol. 183, No. 6
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.6.1983-1989.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chen, S., Bagdasarian, M., Kaufman, M. G., Bates, A. K., Walker, E. D. (2007). Mutational Analysis of the ompA Promoter from Flavobacterium johnsoniae. J. Bacteriol. 189: 5108-5118 [Abstract] [Full Text]  
  • Blanco, M., Gutierrez-Martin, C. B., Rodriguez-Ferri, E. F., Roberts, M. C., Navas, J. (2006). Distribution of Tetracycline Resistance Genes in Actinobacillus pleuropneumoniae Isolates from Spain. Antimicrob. Agents Chemother. 50: 702-708 [Abstract] [Full Text]  
  • Boekema, B. K. H. L., Van Putten, J. P. M., Stockhofe-Zurwieden, N., Smith, H. E. (2004). Host Cell Contact-Induced Transcription of the Type IV Fimbria Gene Cluster of Actinobacillus pleuropneumoniae. Infect. Immun. 72: 691-700 [Abstract] [Full Text]  
  • Mikael, L. G., Pawelek, P. D., Labrie, J., Sirois, M., Coulton, J. W., Jacques, M. (2002). Molecular cloning and characterization of the ferric hydroxamate uptake (fhu) operon in Actinobacillus pleuropneumoniae. Microbiology 148: 2869-2882 [Abstract] [Full Text]  
  • Shea, R. J., Mulks, M. H. (2002). ohr, Encoding an Organic Hydroperoxide Reductase, Is an In Vivo-Induced Gene in Actinobacillus pleuropneumoniae. Infect. Immun. 70: 794-802 [Abstract] [Full Text]  
  • Schneider, T. D. (2001). Strong minor groove base conservation in sequence logos implies DNA distortion or base flipping during replication and transcription initiation. Nucleic Acids Res 29: 4881-4891 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»