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Journal of Bacteriology, March 2001, p. 2137-2140, Vol. 183, No. 6
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.6.2137-2140.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Construction and Characterization of Mutations at Codon 751 of the Escherichia coli gyrB Gene That Confer Resistance to the Antimicrobial Peptide Microcin B17 and Alter the Activity of DNA Gyrase

Francisco J. del Castillo, Ignacio del Castillo, and Felipe Moreno*

Unidad de Genética Molecular, Hospital Ramón y Cajal, 28034-Madrid, Spain

Received 11 October 2000/Accepted 18 December 2000

Microcin B17 is a peptide antibiotic that inhibits DNA replication in Escherichia coli by targeting DNA gyrase. Previously, two independently isolated microcin B17-resistant mutants were shown to harbor the same gyrB point mutation that results in the replacement of tryptophan 751 by arginine in the GyrB polypeptide. We used site-directed mutagenesis to construct mutants in which tryptophan 751 was deleted or replaced by other amino acids. These mutants exhibit altered DNA gyrase activity and different levels of resistance to microcin B17.

* Corresponding author. Mailing address: Unidad de Genética Molecular, Hospital Ramón y Cajal, Carretera de Colmenar Km 9, 28034-Madrid, Spain. Phone: 34-91-3368541. Fax: 34-91-3369016. E-mail: fmoreno{at}hrc.insalud.es.

Journal of Bacteriology, March 2001, p. 2137-2140, Vol. 183, No. 6
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.6.2137-2140.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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