This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goodier, R. I.
Right arrow Articles by Ahmer, B. M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goodier, R. I.
Right arrow Articles by Ahmer, B. M. M.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2001, p. 2249-2258, Vol. 183, No. 7
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.7.2249-2258.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

SirA Orthologs Affect both Motility and Virulence

Robert I. Goodier and Brian M. M. Ahmer*

Department of Microbiology, The Ohio State University, Columbus, Ohio 43210

Received 7 August 2000/Accepted 27 November 2000

The sirA gene of Salmonella enterica serovar Typhimurium encodes a two-component response regulator of the FixJ family that has a positive regulatory influence on the expression of type III secretion genes involved with epithelial cell invasion and the elicitation of bovine gastroenteritis. SirA orthologs in Pseudomonas, Vibrio, and Erwinia control the expression of distinct virulence genes in these genera, but an evolutionarily conserved target of SirA regulation has never been identified. In this study we tested the hypothesis that sirA may be an ancient member of the flagellar regulon. We examined the effect of a sirA mutation on transcriptional fusions to flagellar promoters (flhD, fliE, fliF, flgA, flgB, fliC, fliD, motA, and fliA) while using fusions to the virulence gene sopB as a positive control. SirA had only small regulatory effects on all fusions in liquid medium (less than fivefold). However, in various types of motility agar plates, sirA was able to activate a sopB fusion by up to 63-fold while repressing flagellar fusions by values exceeding 100-fold. Mutations in the sirA orthologs of Escherichia coli, Vibrio cholerae, Pseudomonas fluorescens, and Pseudomonas aeruginosa result in defects in either motility or motility gene regulation, suggesting that control of flagellar regulons may be an evolutionarily conserved function of sirA orthologs. The implications for our understanding of virulence gene regulation in the gamma Proteobacteria are discussed.

* Corresponding author. Mailing address: Department of Microbiology, The Ohio State University, 484 West 12th Ave., Columbus, OH 43210. Phone: (614) 292-1919. Fax: (614) 292-8120. E-mail: ahmer.1{at}osu.edu.

Journal of Bacteriology, April 2001, p. 2249-2258, Vol. 183, No. 7
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.7.2249-2258.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Saini, S., Brown, J. D., Aldridge, P. D., Rao, C. V. (2008). FliZ Is a Posttranslational Activator of FlhD4C2-Dependent Flagellar Gene Expression. J. Bacteriol. 190: 4979-4988 [Abstract] [Full Text]  
  • Hussain, M. B. B. M., Zhang, H.-B., Xu, J.-L., Liu, Q., Jiang, Z., Zhang, L.-H. (2008). The Acyl-Homoserine Lactone-Type Quorum-Sensing System Modulates Cell Motility and Virulence of Erwinia chrysanthemi pv. zeae. J. Bacteriol. 190: 1045-1053 [Abstract] [Full Text]  
  • Lin, D., Rao, C. V., Slauch, J. M. (2008). The Salmonella SPI1 Type Three Secretion System Responds to Periplasmic Disulfide Bond Status via the Flagellar Apparatus and the RcsCDB System. J. Bacteriol. 190: 87-97 [Abstract] [Full Text]  
  • Soscia, C., Hachani, A., Bernadac, A., Filloux, A., Bleves, S. (2007). Cross Talk between Type III Secretion and Flagellar Assembly Systems in Pseudomonas aeruginosa. J. Bacteriol. 189: 3124-3132 [Abstract] [Full Text]  
  • Fink, R. C., Evans, M. R., Porwollik, S., Vazquez-Torres, A., Jones-Carson, J., Troxell, B., Libby, S. J., McClelland, M., Hassan, H. M. (2007). FNR Is a Global Regulator of Virulence and Anaerobic Metabolism in Salmonella enterica Serovar Typhimurium (ATCC 14028s). J. Bacteriol. 189: 2262-2273 [Abstract] [Full Text]  
  • Teplitski, M., Al-Agely, A., Ahmer, B. M. M. (2006). Contribution of the SirA regulon to biofilm formation in Salmonella enterica serovar Typhimurium.. Microbiology 152: 3411-3424 [Abstract] [Full Text]  
  • Herren, C. D., Mitra, A., Palaniyandi, S. K., Coleman, A., Elankumaran, S., Mukhopadhyay, S. (2006). The BarA-UvrY Two-Component System Regulates Virulence in Avian Pathogenic Escherichia coli O78:K80:H9.. Infect. Immun. 74: 4900-4909 [Abstract] [Full Text]  
  • Kay, E., Dubuis, C., Haas, D. (2005). Three small RNAs jointly ensure secondary metabolism and biocontrol in Pseudomonas fluorescens CHA0. Proc. Natl. Acad. Sci. USA 102: 17136-17141 [Abstract] [Full Text]  
  • O'Shea, T. M., DeLoney-Marino, C. R., Shibata, S., Aizawa, S.-I., Wolfe, A. J., Visick, K. L. (2005). Magnesium Promotes Flagellation of Vibrio fischeri. J. Bacteriol. 187: 2058-2065 [Abstract] [Full Text]  
  • Wolfe, A. J. (2005). The Acetate Switch. Microbiol. Mol. Biol. Rev. 69: 12-50 [Abstract] [Full Text]  
  • Kelly, A., Goldberg, M. D., Carroll, R. K., Danino, V., Hinton, J. C. D., Dorman, C. J. (2004). A global role for Fis in the transcriptional control of metabolism and type III secretion in Salmonella enterica serovar Typhimurium. Microbiology 150: 2037-2053 [Abstract] [Full Text]  
  • Whistler, C. A., Ruby, E. G. (2003). GacA Regulates Symbiotic Colonization Traits of Vibrio fischeri and Facilitates a Beneficial Association with an Animal Host. J. Bacteriol. 185: 7202-7212 [Abstract] [Full Text]  
  • Teplitski, M., Goodier, R. I., Ahmer, B. M. M. (2003). Pathways Leading from BarA/SirA to Motility and Virulence Gene Expression in Salmonella. J. Bacteriol. 185: 7257-7265 [Abstract] [Full Text]  
  • Ellermeier, C. D., Slauch, J. M. (2003). RtsA and RtsB Coordinately Regulate Expression of the Invasion and Flagellar Genes in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 185: 5096-5108 [Abstract] [Full Text]  
  • Millikan, D. S., Ruby, E. G. (2003). FlrA, a {sigma}54-Dependent Transcriptional Activator in Vibrio fischeri, Is Required for Motility and Symbiotic Light-Organ Colonization. J. Bacteriol. 185: 3547-3557 [Abstract] [Full Text]  
  • O'Toole, R., Smeulders, M. J., Blokpoel, M. C., Kay, E. J., Lougheed, K., Williams, H. D. (2003). A Two-Component Regulator of Universal Stress Protein Expression and Adaptation to Oxygen Starvation in Mycobacterium smegmatis. J. Bacteriol. 185: 1543-1554 [Abstract] [Full Text]  
  • Smith, J. N., Ahmer, B. M. M. (2003). Detection of Other Microbial Species by Salmonella: Expression of the SdiA Regulon. J. Bacteriol. 185: 1357-1366 [Abstract] [Full Text]  
  • Pernestig, A.-K., Georgellis, D., Romeo, T., Suzuki, K., Tomenius, H., Normark, S., Melefors, O. (2003). The Escherichia coli BarA-UvrY Two-Component System Is Needed for Efficient Switching between Glycolytic and Gluconeogenic Carbon Sources. J. Bacteriol. 185: 843-853 [Abstract] [Full Text]  
  • Millikan, D. S., Ruby, E. G. (2002). Alterations in Vibrio fischeri Motility Correlate with a Delay in Symbiosis Initiation and Are Associated with Additional Symbiotic Colonization Defects. Appl. Environ. Microbiol. 68: 2519-2528 [Abstract] [Full Text]  
  • Michael, B., Smith, J. N., Swift, S., Heffron, F., Ahmer, B. M. M. (2001). SdiA of Salmonella enterica Is a LuxR Homolog That Detects Mixed Microbial Communities. J. Bacteriol. 183: 5733-5742 [Abstract] [Full Text]  
  • Sperandio, V., Torres, A. G., Giron, J. A., Kaper, J. B. (2001). Quorum Sensing Is a Global Regulatory Mechanism in Enterohemorrhagic Escherichia coli O157:H7. J. Bacteriol. 183: 5187-5197 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»