This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wei, Y.
Right arrow Articles by LaRossa, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wei, Y.
Right arrow Articles by LaRossa, R. A.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2001, p. 2259-2264, Vol. 183, No. 7
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.7.2259-2264.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

In Vivo Titration of Mitomycin C Action by Four Escherichia coli Genomic Regions on Multicopy Plasmids

Yan Wei,dagger Amy C. Vollmer,Dagger and Robert A. LaRossa*

Biochemical Science and Engineering, Central Research and Development, DuPont Company, Wilmington, Delaware 19880-0173

Received 6 October 2000/Accepted 3 January 2001

Mitomycin C (MMC), a DNA-damaging agent, is a potent inducer of the bacterial SOS response; surprisingly, it has not been used to select resistant mutants from wild-type Escherichia coli. MMC resistance is caused by the presence of any of four distinct E. coli genes (mdfA, gyrl, rob, and sdiA) on high-copy-number vectors. mdfA encodes a membrane efflux pump whose overexpression results in broad-spectrum chemical resistance. The gyrI (also called sbmC) gene product inhibits DNA gyrase activity in vitro, while the rob protein appears to function in transcriptional activation of efflux pumps. SdiA is a transcriptional activator of ftsQAZ genes involved in cell division.

* Corresponding author. Mailing address: DuPont Company, Central Research and Development, Biochemical Science and Engineering, Experimental Station, P.O. Box 80173, Wilmington, DE 19880-0173. Phone: (302) 695-9264. Fax: (302) 695-9183. E-mail: Robert.A.LaRossa{at}usa.dupont.com.

dagger Present address: Blackstone Technology Group, Boston, MA 02110.

Dagger Present address: Department of Biology, Swarthmore College, Swarthmore, PA 19081-1397.

Journal of Bacteriology, April 2001, p. 2259-2264, Vol. 183, No. 7
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.7.2259-2264.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ghosh, D., Roy, K., Williamson, K. E., Srinivasiah, S., Wommack, K. E., Radosevich, M. (2009). Acyl-Homoserine Lactones Can Induce Virus Production in Lysogenic Bacteria: an Alternative Paradigm for Prophage Induction. Appl. Environ. Microbiol. 75: 7142-7152 [Abstract] [Full Text]  
  • Lee, J., Maeda, T., Hong, S. H., Wood, T. K. (2009). Reconfiguring the Quorum-Sensing Regulator SdiA of Escherichia coli To Control Biofilm Formation via Indole and N-Acylhomoserine Lactones. Appl. Environ. Microbiol. 75: 1703-1716 [Abstract] [Full Text]  
  • Yeager, C. M., Bottomley, P. J., Arp, D. J. (2001). Requirement of DNA Repair Mechanisms for Survival of Burkholderia cepacia G4 upon Degradation of Trichloroethylene. Appl. Environ. Microbiol. 67: 5384-5391 [Abstract] [Full Text]  
  • Smulski, D. R., Huang, L. L., McCluskey, M. P., Reeve, M. J. G., Vollmer, A. C., Van Dyk, T. K., LaRossa, R. A. (2001). Combined, Functional Genomic-Biochemical Approach to Intermediary Metabolism: Interaction of Acivicin, a Glutamine Amidotransferase Inhibitor, with Escherichia coli K-12. J. Bacteriol. 183: 3353-3364 [Abstract] [Full Text]  
  • Wei, Y., Lee, J.-M., Smulski, D. R., LaRossa, R. A. (2001). Global Impact of sdiA Amplification Revealed by Comprehensive Gene Expression Profiling of Escherichia coli. J. Bacteriol. 183: 2265-2272 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»