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Journal of Bacteriology, April 2001, p. 2662-2666, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2662-2666.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

An alpha /beta -Type, Small, Acid-Soluble Spore Protein Which Has Very High Affinity for DNA Prevents Outgrowth of Bacillus subtilis Spores

Christopher S. Hayesdagger and Peter Setlow*

Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030

Received 31 October 2000/Accepted 26 January 2001

A derivative of SspC, a minor alpha /beta -type, small, acid-soluble spore protein (SASP) from Bacillus subtilis, was generated that has a very high affinity for DNA. This protein (SspCDelta 11-D13K) was able to confer UV resistance on spores lacking alpha /beta -type SASP, and spores with SspCDelta 11-D13K triggered germination normally. However, SspCDelta 11-D13K blocked outgrowth of >= 90% of germinated spores, and SspCDelta 11-D13K persisted in these germinated spores, whereas wild-type SspC was almost completely degraded. The outgrowth phenotype of spores with SspCDelta 11-D13K is proposed to be due to the high stability of the SspCDelta 11-D13K-DNA complex, which prevents rapid degradation of this alpha /beta -type SASP early in germination. The persistence of this protein on spore DNA then interferes with transcription during spore outgrowth.

* Corresponding author. Mailing address: Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06030. Phone: (860) 679-2607. Fax: (860) 679-3408. E-mail: setlow{at}sun.uchc.edu.

dagger Present address: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.

Journal of Bacteriology, April 2001, p. 2662-2666, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2662-2666.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kosman, J., Setlow, P. (2003). Effects of Carboxy-Terminal Modifications and pH on Binding of a Bacillus subtilis Small, Acid-Soluble Spore Protein to DNA. J. Bacteriol. 185: 6095-6103 [Abstract] [Full Text]  
  • Hathout, Y., Setlow, B., Cabrera-Martinez, R.-M., Fenselau, C., Setlow, P. (2003). Small, Acid-Soluble Proteins as Biomarkers in Mass Spectrometry Analysis of Bacillus Spores. Appl. Environ. Microbiol. 69: 1100-1107 [Abstract] [Full Text]  


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