This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Härtl, B.
Right arrow Articles by Schumann, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Härtl, B.
Right arrow Articles by Schumann, W.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2001, p. 2696-2699, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2696-2699.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Development of a New Integration Site within the Bacillus subtilis Chromosome and Construction of Compatible Expression Cassettes

Barbara Härtl, Wolfgang Wehrl, Thomas Wiegert, Georg Homuth, and Wolfgang Schumann*

Institute of Genetics, University of Bayreuth, D-95440 Bayreuth, Germany

Received 16 October 2000/Accepted 8 January 2001

The Bacillus subtilis lacA gene, coding for beta -galactosidase, has been explored as a new site able to accept DNA sequences from nonreplicating delivery vectors. Two such delivery expression vectors have been constructed and shown to be useful in obtaining regulated expression from the chromosomal location. In another experiment, it was shown that the integration of a regulatory gene at the lacA locus was able to control the expression of a transcriptional fusion at the amyE locus. These experiments demonstrate that both integration sites can be used simultaneously to obtain regulated expression of desired genes.

* Corresponding author. Mailing address: Institute of Genetics, University of Bayreuth, D-95440 Bayreuth, Germany. Phone: 49 (921) 552708. Fax: 49 (921) 552710. E-mail: wolfgang.schumann{at}uni-bayreuth.de.

Journal of Bacteriology, April 2001, p. 2696-2699, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2696-2699.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Fickers, P., Guez, J.-S., Damblon, C., Leclere, V., Bechet, M., Jacques, P., Joris, B. (2009). High-Level Biosynthesis of the Anteiso-C17 Isoform of the Antibiotic Mycosubtilin in Bacillus subtilis and Characterization of Its Candidacidal Activity. Appl. Environ. Microbiol. 75: 4636-4640 [Abstract] [Full Text]  
  • Eijlander, R. T., Kolbusz, M. A., Berendsen, E. M., Kuipers, O. P. (2009). Effects of altered TatC proteins on protein secretion efficiency via the twin-arginine translocation pathway of Bacillus subtilis. Microbiology 155: 1776-1785 [Abstract] [Full Text]  
  • Andre, G., Even, S., Putzer, H., Burguiere, P., Croux, C., Danchin, A., Martin-Verstraete, I., Soutourina, O. (2008). S-box and T-box riboswitches and antisense RNA control a sulfur metabolic operon of Clostridium acetobutylicum. Nucleic Acids Res 36: 5955-5969 [Abstract] [Full Text]  
  • Yan, X., Yu, H.-J., Hong, Q., Li, S.-P. (2008). Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis. Appl. Environ. Microbiol. 74: 5556-5562 [Abstract] [Full Text]  
  • Heinrich, J., Lunden, T., Kontinen, V. P., Wiegert, T. (2008). The Bacillus subtilis ABC transporter EcsAB influences intramembrane proteolysis through RasP. Microbiology 154: 1989-1997 [Abstract] [Full Text]  
  • Vlamakis, H., Aguilar, C., Losick, R., Kolter, R. (2008). Control of cell fate by the formation of an architecturally complex bacterial community. Genes Dev. 22: 945-953 [Abstract] [Full Text]  
  • Nakano, S., Zheng, G., Nakano, M. M., Zuber, P. (2002). Multiple Pathways of Spx (YjbD) Proteolysis in Bacillus subtilis. J. Bacteriol. 184: 3664-3670 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»