This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blokesch, M.
Right arrow Articles by Böck, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blokesch, M.
Right arrow Articles by Böck, A.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2001, p. 2817-2822, Vol. 183, No. 9
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.9.2817-2822.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Interplay between the Specific Chaperone-Like Proteins HybG and HypC in Maturation of Hydrogenases 1, 2, and 3 from Escherichia coli

Melanie Blokesch, Axel Magalon, and August Böck*

Lehrstuhl für Mikrobiologie der Universität München, D-80638 Munich, Germany

Received 4 December 2000/Accepted 9 February 2001

The hybG gene product from Escherichia coli has been identified as a chaperone-like protein acting in the maturation of hydrogenases 1 and 2. It was shown that HybG forms a complex with the precursor of the large subunit of hydrogenase 2. As with HypC, which is the chaperone-like protein involved in hydrogenase 3 maturation, the N-terminal cysteine residue is crucial for complex formation. Introduction of a deletion into hybG abolished the generation of active hydrogenase 2 but only quantitatively reduced hydrogenase 1 activity since HypC could replace HybG in this function. In contrast, HybG could not take over the role of HypC in a Delta hypC genetic background. Overproduction of HybG, especially of the variants with the replaced N-terminal cysteine residue, strongly interfered with hydrogenase 3 maturation, apparently by titrating some other component(s) of the maturation machinery. The results indicate that the three hydrogenase isoenzymes not only are interacting at the functional level but are also interconnected during the maturation process.

* Corresponding author. Mailing address: Institute of Genetics and Microbiology, University of Munich, Maria-Ward-Strasse 1a, D-80638 Munich, Germany. Phone: 49-89-21806120. Fax: 49-89-21806122. E-mail: august.boeck{at}lrz.uni-muenchen.de.

Journal of Bacteriology, May 2001, p. 2817-2822, Vol. 183, No. 9
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.9.2817-2822.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Maroti, G., Tong, Y., Yooseph, S., Baden-Tillson, H., Smith, H. O., Kovacs, K. L., Frazier, M., Venter, J. C., Xu, Q. (2009). Discovery of [NiFe] Hydrogenase Genes in Metagenomic DNA: Cloning and Heterologous Expression in Thiocapsa roseopersicina. Appl. Environ. Microbiol. 75: 5821-5830 [Abstract] [Full Text]  
  • Ludwig, M., Schubert, T., Zebger, I., Wisitruangsakul, N., Saggu, M., Strack, A., Lenz, O., Hildebrandt, P., Friedrich, B. (2009). Concerted Action of Two Novel Auxiliary Proteins in Assembly of the Active Site in a Membrane-bound [NiFe] Hydrogenase. J. Biol. Chem. 284: 2159-2168 [Abstract] [Full Text]  
  • Blokesch, M., Rohrmoser, M., Rode, S., Bock, A. (2004). HybF, a Zinc-Containing Protein Involved in NiFe Hydrogenase Maturation. J. Bacteriol. 186: 2603-2611 [Abstract] [Full Text]  
  • Fodor, B. D., Kovacs, A. T., Csaki, R., Hunyadi-Gulyas, E., Klement, E., Maroti, G., Meszaros, L. S., Medzihradszky, K. F., Rakhely, G., Kovacs, K. L. (2004). Modular Broad-Host-Range Expression Vectors for Single-Protein and Protein Complex Purification. Appl. Environ. Microbiol. 70: 712-721 [Abstract] [Full Text]  
  • Self, W. T., Hasona, A., Shanmugam, K. T. (2004). Expression and Regulation of a Silent Operon, hyf, Coding for Hydrogenase 4 Isoenzyme in Escherichia coli. J. Bacteriol. 186: 580-587 [Abstract] [Full Text]  
  • Hube, M., Blokesch, M., Bock, A. (2002). Network of Hydrogenase Maturation in Escherichia coli: Role of Accessory Proteins HypA and HybF. J. Bacteriol. 184: 3879-3885 [Abstract] [Full Text]  
  • Tamagnini, P., Axelsson, R., Lindberg, P., Oxelfelt, F., Wunschiers, R., Lindblad, P. (2002). Hydrogenases and Hydrogen Metabolism of Cyanobacteria. Microbiol. Mol. Biol. Rev. 66: 1-20 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»