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Journal of Bacteriology, May 2001, p. 2943-2946, Vol. 183, No. 9
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.9.2943-2946.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of Intragenic Recombination in a Hyperthermophilic Archaeon via Conjugational DNA Exchange

Michelle S. Reilly and Dennis W. Grogan*

Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio 54221-0006

Received 27 November 2000/Accepted 14 February 2001

Sulfolobus acidocaldarius is so far the only hyperthermophilic archaeon in which genetic recombination can be assayed by conjugation and simple selections. Crosses among spontanteous pyr mutants were able to resolve closely spaced chromosomal mutations, identify deletions and rearrangements, and map mutations to a given deletion interval. Frameshift mutations in pyrE exerted polar effects that depressed orotidine-5'-monophosphate decarboxylase activity (encoded by pyrF), whereas base pair substitutions and an 18-bp deletion had no effect.

* Corresponding author. Mailing address: Department of Biological Sciences, P.O. Box 210006, Cincinnati, OH 54221-0006. Phone: (513) 556-9748. Fax: (513) 556-5299. E-mail: grogandw{at}emailuc.edu.

Journal of Bacteriology, May 2001, p. 2943-2946, Vol. 183, No. 9
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.9.2943-2946.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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