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Journal of Bacteriology, July 2002, p. 3560-3568, Vol. 184, No. 13
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.13.3560-3568.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The Rhizobium etli cyaC Product: Characterization of a Novel Adenylate Cyclase Class

Juan Téllez-Sosa,1 Nora Soberón,1 Alicia Vega-Segura,2 María E. Torres-Márquez,2 and Miguel A. Cevallos1*

Programa de Evolución Molecular, Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, C.P. 62210, Cuernavaca, Morelos,1 Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, C.P. 04510, Distrito Federal, México2

Received 18 December 2001/ Accepted 7 April 2002

Adenylate cyclases (ACs) catalyze the formation of 3',5'-cyclic AMP (cAMP) from ATP. A novel AC-encoding gene, cyaC, was isolated from Rhizobium etli by phenotypic complementation of an Escherichia coli cya mutant. The functionality of the cyaC gene was corroborated by its ability to restore cAMP accumulation in an E. coli cya mutant. Further, overexpression of a malE::cyaC fusion protein allowed the detection of significant AC activity levels in cell extracts of an E. coli cya mutant. CyaC is unrelated to any known AC or to any other protein exhibiting a currently known function. Thus, CyaC represents the first member of a novel class of ACs (class VI). Hypothetical genes of unknown function similar to cyaC have been identified in the genomes of the related bacterial species Mesorhizobium loti, Sinorhizobium meliloti, and Agrobacterium tumefaciens. The cyaC gene is cotranscribed with a gene similar to ohr of Xanthomonas campestris and is expressed only in the presence of organic hydroperoxides. The physiological performance of an R. etli cyaC mutant was indistinguishable from that of the wild-type parent strain both under free-living conditions and during symbiosis.

* Corresponding author. Mailing address: Programa de Evolución Molecular, Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, A. P. 565-A, C. P. 62210, Cuernavaca, Morelos, México. Phone: 52(777)3114663. Fax: 52(777)3175581. E-mail: mac{at}cifn.unam.mx.

Journal of Bacteriology, July 2002, p. 3560-3568, Vol. 184, No. 13
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.13.3560-3568.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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