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Journal of Bacteriology, July 2002, p. 3699-3703, Vol. 184, No. 13
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.13.3699-3703.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Toxic Waste Disposal in Escherichia coli

Robert B. Helling,* Brian K. Janes, Heather Kimball, Timothy Tran, Michael Bundesmann, Pietra Check, Darcy Phelan, and Charles Miller

Department of Biology, University of Michigan, Ann Arbor, Michigan 48109-1048

Received 22 November 2000/ Accepted 2 April 2002

About 10% of the nalidixic acid-resistant (Nalr) mutants in a transposition-induced library exhibited a growth factor requirement as the result of cysH, icdA, metE, or purB mutation. Resistance in all of these mutants required a functional AcrAB-TolC efflux pump, but the EmrAB-TolC pump played no obvious role. Transcription of acrAB was increased in each type of Nalr mutant. In the icdA and purB mutants, each of the known signaling pathways appeared to be used in activating the AcrAB-TolC pump. The metabolites that accumulate upstream of the blocks caused by the mutations are hypothesized to increase the levels of the AcrAB-TolC pump, thereby removing nalidixic acid from the organism.

* Corresponding author. Mailing address: Department of Biology, University of Michigan, Natural Science Building, 830 N. University, Ann Arbor, MI 48109-1048. Phone: (734) 764-1455. Fax: (734) 647-0884. E-mail: helling{at}umich.edu.

Journal of Bacteriology, July 2002, p. 3699-3703, Vol. 184, No. 13
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.13.3699-3703.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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