This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ouchane, S.
Right arrow Articles by Astier, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ouchane, S.
Right arrow Articles by Astier, C.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2002, p. 3815-3822, Vol. 184, No. 14
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.14.3815-3822.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Natural Resistance to Inhibitors of the Ubiquinol Cytochrome c Oxidoreductase of Rubrivivax gelatinosus: Sequence and Functional Analysis of the Cytochrome bc1 Complex

Soufian Ouchane, Ileana Agalidis, and Chantal Astier*

Centre de Génétique Moléculaire CNRS (UPR-2167) Associé à l'Université Pierre et Marie Curie, 91198 Gif sur Yvette Cedex, France

Received 26 March 2002/ Accepted 22 April 2002

Biochemical analyses of Rubrivivax gelatinosus membranes have revealed that the cytochrome bc1 complex is highly resistant to classical inhibitors including myxothiazol, stigmatellin, and antimycin. This is the first report of a strain exhibiting resistance to inhibitors of both catalytic Q0 and Qi sites. Because the resistance to cytochrome bc1 inhibitors is primarily related to the cytochrome b primary structure, the petABC operon encoding the subunits of the cytochrome bc1 complex of Rubrivivax gelatinosus was sequenced. In addition to homologies to the corresponding proteins from other organisms, the deduced amino acid sequence of the cytochrome b polypeptide shows (i) an E303V substitution in the highly conserved PEWY loop involved in quinol/stigmatellin binding, (ii) other substitutions that could be involved in resistance to cytochrome bc1 inhibitors, and (iii) 14 residues instead of 13 between the histidines in helix IV that likely serve as the second axial ligand to the bH and bL hemes, respectively. These characteristics imply different functional properties of the cytochrome bc1 complex of this bacterium. The consequences of these structural features for the resistance to inhibitors and for the properties of R. gelatinosus cytochrome bc1 are discussed with reference to the structure and function of the cytochrome bc1 complexes from other organisms.

* Corresponding author. Mailing address: Centre de Génétique Moléculaire CNRS (UPR-2167) Associé à l'Université Pierre et Marie Curie, BÂt. 24, Ave. de la Terrasse, 91198 Gif sur Yvette Cedex, France. Phone: 33-1-69823137. Fax: 33-1-69823230. E-mail: astier{at}cgm.cnrs-gif.fr.

Journal of Bacteriology, July 2002, p. 3815-3822, Vol. 184, No. 14
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.14.3815-3822.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hong, Y., Xu, M., Guo, J., Xu, Z., Chen, X., Sun, G. (2007). Respiration and Growth of Shewanella decolorationis S12 with an Azo Compound as the Sole Electron Acceptor. Appl. Environ. Microbiol. 73: 64-72 [Abstract] [Full Text]  
  • Rossier, O., Cianciotto, N. P. (2005). The Legionella pneumophila tatB Gene Facilitates Secretion of Phospholipase C, Growth under Iron-Limiting Conditions, and Intracellular Infection. Infect. Immun. 73: 2020-2032 [Abstract] [Full Text]  
  • Steunou, A.-S., Astier, C., Ouchane, S. (2004). Regulation of Photosynthesis Genes in Rubrivivax gelatinosus: Transcription Factor PpsR Is Involved in both Negative and Positive Control. J. Bacteriol. 186: 3133-3142 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»