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Journal of Bacteriology, July 2002, p. 3834-3838, Vol. 184, No. 14
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.14.3834-3838.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

DNA Polymerases of Low-GC Gram-Positive Eubacteria: Identification of the Replication-Specific Enzyme Encoded by dnaE

Marjorie H. Barnes,,{dagger} Shelley D. Miller,,{ddagger} and Neal C. Brown*

Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655

Received 30 January 2002/ Accepted 19 April 2002

dnaE, the gene encoding one of the two replication-specific DNA polymerases (Pols) of low-GC-content gram-positive bacteria (E. Dervyn et al., Science 294:1716-1719, 2001; R. Inoue et al., Mol. Genet. Genomics 266:564-571, 2001), was cloned from Bacillus subtilis, a model low-GC gram-positive organism. The gene was overexpressed in Escherichia coli. The purified recombinant product displayed inhibitor responses and physical, catalytic, and antigenic properties indistinguishable from those of the low-GC gram-positive-organism-specific enzyme previously named DNA Pol II after the polB-encoded DNA Pol II of E. coli. Whereas a polB-like gene is absent from low-GC gram-positive genomes and whereas the low-GC gram-positive DNA Pol II strongly conserves a dnaE-like, Pol III primary structure, it is proposed that it be renamed DNA polymerase III E (Pol III E) to accurately reflect its replicative function and its origin from dnaE. It is also proposed that DNA Pol III, the other replication-specific Pol of low-GC gram-positive organisms, be renamed DNA polymerase III C (Pol III C) to denote its origin from polC. By this revised nomenclature, the DNA Pols that are expressed constitutively in low-GC gram-positive bacteria would include DNA Pol I, the dispensable repair enzyme encoded by polA, and the two essential, replication-specific enzymes Pol III C and Pol III E, encoded, respectively, by polC and dnaE.

* Corresponding author. Present address: GL Synthesis, One Innovation Dr., Worcester, MA 01605. Phone: (508) 754-6700. Fax: (508) 754-7075. E-mail: neal.brown{at}glsynthesis.com.

{dagger} Present address: Microbiotix, Inc., Worcester, MA 01605.

{ddagger} Present address: Hollister, Inc., Stuarts Draft, VA 24477.

Journal of Bacteriology, July 2002, p. 3834-3838, Vol. 184, No. 14
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.14.3834-3838.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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