This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baranova, N.
Right arrow Articles by Nikaido, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baranova, N.
Right arrow Articles by Nikaido, H.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 2002, p. 4168-4176, Vol. 184, No. 15
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.15.4168-4176.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The BaeSR Two-Component Regulatory System Activates Transcription of the yegMNOB (mdtABCD) Transporter Gene Cluster in Escherichia coli and Increases Its Resistance to Novobiocin and Deoxycholate

Natalya Baranova and Hiroshi Nikaido*

Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3206

Received 8 March 2002/ Accepted 1 May 2002

Screening of random fragments of Escherichia coli genomic DNA for their ability to increase the novobiocin resistance of a hypersusceptible {Delta}acrAB mutant resulted in the isolation of a plasmid containing baeR, which codes for the response regulator of the two-component regulatory system BaeSR. When induced for expression, baeR cloned in multicopy plasmid pTrc99A significantly increased the resistance of the {Delta}acrAB host strain to novobiocin (16-fold) and to deoxycholate (8-fold). Incubation of cells with novobiocin followed by a chromatographic assay for intracellular drug showed that overproduced BaeR decreased drastically the drug accumulation, presumably via increased active efflux. The genes baeSR are part of a putative operon, yegMNOB baeSR. Direct binding of BaeR to the yegM promoter was demonstrated in vitro by gel retardation assay. The gene yegB, which codes for a major facilitator superfamily transporter, was not necessary for increased resistance, but deletion of yegO or an in-frame deletion of yegN, both of which code for resistance-nodulation-cell division-type multidrug transporters, abolished the BaeR-induced increase in resistance. It is likely that both YegN and YegO produce a complex(es) with the membrane fusion protein family member YegM and pump out novobiocin and deoxycholate. We accordingly propose to rename yegMNOB as mdtABCD (mdt for multidrug transporter). Finally, the expression of two other genes, yicO and ygcL, was shown to be regulated by BaeR, but it is not known if they play any roles in resistance.

* Corresponding author. Mailing address: Room 229, Stanley Hall, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3206. Phone: (510) 642-2027. Fax: (510) 643-9290. E-mail: nhiroshi{at}uclink4.berkeley.edu.

Journal of Bacteriology, August 2002, p. 4168-4176, Vol. 184, No. 15
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.15.4168-4176.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mima, T., Kohira, N., Li, Y., Sekiya, H., Ogawa, W., Kuroda, T., Tsuchiya, T. (2009). Gene cloning and characteristics of the RND-type multidrug efflux pump MuxABC-OpmB possessing two RND components in Pseudomonas aeruginosa. Microbiology 155: 3509-3517 [Abstract] [Full Text]  
  • Shimada, T., Yamamoto, K., Ishihama, A. (2009). Involvement of the Leucine Response Transcription Factor LeuO in Regulation of the Genes for Sulfa Drug Efflux. J. Bacteriol. 191: 4562-4571 [Abstract] [Full Text]  
  • Price, N. L., Raivio, T. L. (2009). Characterization of the Cpx Regulon in Escherichia coli Strain MC4100. J. Bacteriol. 191: 1798-1815 [Abstract] [Full Text]  
  • Zoetendal, E. G., Smith, A. H., Sundset, M. A., Mackie, R. I. (2008). The BaeSR Two-Component Regulatory System Mediates Resistance to Condensed Tannins in Escherichia coli. Appl. Environ. Microbiol. 74: 535-539 [Abstract] [Full Text]  
  • Nishino, K., Nikaido, E., Yamaguchi, A. (2007). Regulation of Multidrug Efflux Systems Involved in Multidrug and Metal Resistance of Salmonella enterica Serovar Typhimurium. J. Bacteriol. 189: 9066-9075 [Abstract] [Full Text]  
  • Mima, T., Joshi, S., Gomez-Escalada, M., Schweizer, H. P. (2007). Identification and Characterization of TriABC-OpmH, a Triclosan Efflux Pump of Pseudomonas aeruginosa Requiring Two Membrane Fusion Proteins. J. Bacteriol. 189: 7600-7609 [Abstract] [Full Text]  
  • Carlsson, K. E., Liu, J., Edqvist, P. J., Francis, M. S. (2007). Extracytoplasmic-Stress-Responsive Pathways Modulate Type III Secretion in Yersinia pseudotuberculosis. Infect. Immun. 75: 3913-3924 [Abstract] [Full Text]  
  • Bohnert, J. A., Schuster, S., Fahnrich, E., Trittler, R., Kern, W. V. (2007). Altered spectrum of multidrug resistance associated with a single point mutation in the Escherichia coli RND-type MDR efflux pump YhiV (MdtF). J Antimicrob Chemother 59: 1216-1222 [Abstract] [Full Text]  
  • Stavri, M., Piddock, L. J. V., Gibbons, S. (2007). Bacterial efflux pump inhibitors from natural sources. J Antimicrob Chemother 59: 1247-1260 [Abstract] [Full Text]  
  • Elkins, C. A., Mullis, L. B. (2007). Substrate Competition Studies Using Whole-Cell Accumulation Assays with the Major Tripartite Multidrug Efflux Pumps of Escherichia coli. Antimicrob. Agents Chemother. 51: 923-929 [Abstract] [Full Text]  
  • Depardieu, F., Podglajen, I., Leclercq, R., Collatz, E., Courvalin, P. (2007). Modes and Modulations of Antibiotic Resistance Gene Expression. Clin. Microbiol. Rev. 20: 79-114 [Abstract] [Full Text]  
  • Schlicht, M., Berens, C., Daam, J., Hillen, W. (2006). Random Insertion of a TetR-Inducing Peptide Tag into Escherichia coli Proteins Allows Analysis of Protein Levels by Induction of Reporter Gene Expression. Appl. Environ. Microbiol. 72: 5637-5642 [Abstract] [Full Text]  
  • Froelich, J. M., Tran, K., Wall, D. (2006). A pmrA Constitutive Mutant Sensitizes Escherichia coli to Deoxycholic Acid. J. Bacteriol. 188: 1180-1183 [Abstract] [Full Text]  
  • Elkins, C. A., Mullis, L. B. (2006). Mammalian Steroid Hormones Are Substrates for the Major RND- and MFS-Type Tripartite Multidrug Efflux Pumps of Escherichia coli. J. Bacteriol. 188: 1191-1195 [Abstract] [Full Text]  
  • Hu, W. S., Li, P.-C., Cheng, C.-Y. (2005). Correlation between Ceftriaxone Resistance of Salmonella enterica Serovar Typhimurium and Expression of Outer Membrane Proteins OmpW and Ail/OmpX-Like Protein, Which Are Regulated by BaeR of a Two-Component System. Antimicrob. Agents Chemother. 49: 3955-3958 [Abstract] [Full Text]  
  • Poole, K. (2005). Efflux-mediated antimicrobial resistance. J Antimicrob Chemother 56: 20-51 [Abstract] [Full Text]  
  • Raphael, B. H., Pereira, S., Flom, G. A., Zhang, Q., Ketley, J. M., Konkel, M. E. (2005). The Campylobacter jejuni Response Regulator, CbrR, Modulates Sodium Deoxycholate Resistance and Chicken Colonization. J. Bacteriol. 187: 3662-3670 [Abstract] [Full Text]  
  • Lin, J., Akiba, M., Sahin, O., Zhang, Q. (2005). CmeR Functions as a Transcriptional Repressor for the Multidrug Efflux Pump CmeABC in Campylobacter jejuni. Antimicrob. Agents Chemother. 49: 1067-1075 [Abstract] [Full Text]  
  • Nishino, K., Honda, T., Yamaguchi, A. (2005). Genome-Wide Analyses of Escherichia coli Gene Expression Responsive to the BaeSR Two-Component Regulatory System. J. Bacteriol. 187: 1763-1772 [Abstract] [Full Text]  
  • Lee, L. J., Barrett, J. A., Poole, R. K. (2005). Genome-Wide Transcriptional Response of Chemostat-Cultured Escherichia coli to Zinc. J. Bacteriol. 187: 1124-1134 [Abstract] [Full Text]  
  • Marchand, I., Damier-Piolle, L., Courvalin, P., Lambert, T. (2004). Expression of the RND-Type Efflux Pump AdeABC in Acinetobacter baumannii Is Regulated by the AdeRS Two-Component System. Antimicrob. Agents Chemother. 48: 3298-3304 [Abstract] [Full Text]  
  • Hirakawa, H., Nishino, K., Yamada, J., Hirata, T., Yamaguchi, A. (2003). {beta}-Lactam resistance modulated by the overexpression of response regulators of two-component signal transduction systems in Escherichia coli. J Antimicrob Chemother 52: 576-582 [Abstract] [Full Text]  
  • Elkins, C. A., Nikaido, H. (2003). Chimeric Analysis of AcrA Function Reveals the Importance of Its C-Terminal Domain in Its Interaction with the AcrB Multidrug Efflux Pump. J. Bacteriol. 185: 5349-5356 [Abstract] [Full Text]  
  • Rojas, A., Segura, A., Guazzaroni, M. E., Teran, W., Hurtado, A., Gallegos, M. T., Ramos, J. L. (2003). In Vivo and In Vitro Evidence that TtgV Is the Specific Regulator of the TtgGHI Multidrug and Solvent Efflux Pump of Pseudomonas putida. J. Bacteriol. 185: 4755-4763 [Abstract] [Full Text]  
  • Zhou, L., Lei, X.-H., Bochner, B. R., Wanner, B. L. (2003). Phenotype MicroArray Analysis of Escherichia coli K-12 Mutants with Deletions of All Two-Component Systems. J. Bacteriol. 185: 4956-4972 [Abstract] [Full Text]  
  • Hirakawa, H., Nishino, K., Hirata, T., Yamaguchi, A. (2003). Comprehensive Studies of Drug Resistance Mediated by Overexpression of Response Regulators of Two-Component Signal Transduction Systems in Escherichia coli. J. Bacteriol. 185: 1851-1856 [Abstract] [Full Text]  
  • Grkovic, S., Brown, M. H., Skurray, R. A. (2002). Regulation of Bacterial Drug Export Systems. Microbiol. Mol. Biol. Rev. 66: 671-701 [Abstract] [Full Text]  
  • Nagakubo, S., Nishino, K., Hirata, T., Yamaguchi, A. (2002). The Putative Response Regulator BaeR Stimulates Multidrug Resistance of Escherichia coli via a Novel Multidrug Exporter System, MdtABC. J. Bacteriol. 184: 4161-4167 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»