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Journal of Bacteriology, August 2002, p. 4626-4629, Vol. 184, No. 16
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.16.4626-4629.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Recombination-Promoting Activity of the Bacteriophage {lambda} Rap Protein in Escherichia coli K-12

Anthony R. Poteete,* Anita C. Fenton, and Hsinju R. Wang

Department of Molecular Genetics & Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655

Received 23 October 2001/ Accepted 9 May 2002

The rap gene of bacteriophage {lambda} was placed in the chromosome of an Escherichia coli K-12 strain in which the recBCD gene cluster had previously been replaced by the {lambda} red genes and in which the recG gene had been deleted. Recombination between linear double-stranded DNA molecules and the chromosome was tested in variants of the recG{Delta} red+ rap+ strain bearing mutations in genes known to affect recombination in other cellular pathways. The linear DNA was a 4-kb fragment containing the cat gene, with flanking lac sequences, released from an infecting phage chromosome by restriction enzyme cleavage in the cell. Replacement of wild-type lacZ with lacZ::cat was monitored by measuring the production of Lac-deficient chloramphenicol-resistant bacterial progeny. The results of these experiments indicated that the {lambda} rap gene could functionally substitute for the E. coli ruvC gene in Red-mediated recombination.

* Corresponding author. Mailing address: Department of Molecular Genetics & Microbiology, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA 01655. Phone: (508) 856-3708. Fax: (508) 856-5920. E-mail: anthony.poteete{at}umassmed.edu.

Journal of Bacteriology, August 2002, p. 4626-4629, Vol. 184, No. 16
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.16.4626-4629.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hayes, S., Asai, K., Chu, A. M., Hayes, C. (2005). NinR- and Red-Mediated Phage-Prophage Marker Rescue Recombination in Escherichia coli: Recovery of a Nonhomologous imm{lambda} DNA Segment by Infecting {lambda}imm434 Phages. Genetics 170: 1485-1499 [Abstract] [Full Text]  
  • Poteete, A. R. (2004). Modulation of DNA Repair and Recombination by the Bacteriophage {lambda} Orf Function in Escherichia coli K-12. J. Bacteriol. 186: 2699-2707 [Abstract] [Full Text]  


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