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Journal of Bacteriology, August 2002, p. 4630-4635, Vol. 184, No. 16
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.16.4630-4635.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genome-Wide Transcriptional Profiling in a Histidine Kinase Mutant of Helicobacter pylori Identifies Members of a Regulon

Mark H. Forsyth,1* Ping Cao,2 Preston P. Garcia,1 Joshua D. Hall,1 and Timothy L. Cover2,3

Department of Biology, The College of William and Mary, Williamsburg, Virginia 23187,1 Department of Medicine and Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232,2 Veterans Affairs Medical Center, Nashville, Tennessee 372123

Received 10 December 2001/ Accepted 21 May 2002

To identify putative members of a regulon controlled by the H. pylori sensory histidine kinase HP0164 (HK0164), we constructed HK0164 null mutant H. pylori strains and analyzed bacterial gene transcription using DNA arrays. Seven genes were differentially expressed in multiple HK0164 mutant strains compared to their expression in control strains. Strain-dependent variations in differential expression were also detected. These results indicate that the signal transduction circuit utilizing HK0164 controls the transcription of at least seven genes in H. pylori.

* Corresponding author: Biology Department, Millington Hall, The College of William and Mary, Williamsburg, VA 23187. Phone: (757) 221-2489. Fax: (757) 221-6483. E-mail: mhfors{at}wm.edu.

Journal of Bacteriology, August 2002, p. 4630-4635, Vol. 184, No. 16
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.16.4630-4635.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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