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Journal of Bacteriology, September 2002, p. 4920-4924, Vol. 184, No. 17
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.17.4920-4924.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Membrane Association and Kinase-Like Motifs of the RamC Protein of Streptomyces coelicolor

Michael E. Hudson, Dachuan Zhang, and Justin R. Nodwell*

Department of Biochemistry and Antimicrobial Research Centre, McMaster University, Hamilton, Ontario L8N 3Z5, Canada

Received 15 March 2002/ Accepted 10 June 2002

The protein RamC is required for the production of the spore-forming cells called aerial hyphae by the filamentous bacterium Streptomyces coelicolor. We showed that RamC, which contains several weakly predicted membrane-spanning sequences, is located exclusively in the S. coelicolor membrane. By constructing site-directed mutants in the cloned ramC gene and complementing a ramC null mutant, we showed that protein kinase-like sequence motifs in the amino-terminal half of the protein are required for function in vivo. These data suggest that RamC is a membrane-associated receptor kinase.

* Corresponding author. Mailing address: Department of Biochemistry and Antimicrobial Research Centre, McMaster University, 1200 Main St. W, Hamilton, Ontario L8N 3Z5, Canada. Phone: (905) 525-9140, ext. 27335. Fax: (905) 522-9033. E-mail: nodwellj{at}mcmaster.ca.

Journal of Bacteriology, September 2002, p. 4920-4924, Vol. 184, No. 17
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.17.4920-4924.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kodani, S., Hudson, M. E., Durrant, M. C., Buttner, M. J., Nodwell, J. R., Willey, J. M. (2004). From The Cover: The SapB morphogen is a lantibiotic-like peptide derived from the product of the developmental gene ramS in Streptomyces coelicolor. Proc. Natl. Acad. Sci. USA 101: 11448-11453 [Abstract] [Full Text]  
  • Hudson, M. E., Nodwell, J. R. (2004). Dimerization of the RamC Morphogenetic Protein of Streptomyces coelicolor. J. Bacteriol. 186: 1330-1336 [Abstract] [Full Text]  
  • Petrickova, K., Petricek, M. (2003). Eukaryotic-type protein kinases in Streptomyces coelicolor: variations on a common theme. Microbiology 149: 1609-1621 [Abstract] [Full Text]  
  • Kroos, L., Maddock, J. R. (2003). Prokaryotic Development: Emerging Insights. J. Bacteriol. 185: 1128-1146 [Full Text]  


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