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Journal of Bacteriology, September 2002, p. 4925-4929, Vol. 184, No. 17
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.17.4925-4929.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The Membrane-Bound {alpha}-Glucuronidase from Pseudomonas cellulosa Hydrolyzes 4-O-Methyl-D-Glucuronoxylooligosaccharides but Not 4-O-Methyl-D-Glucuronoxylan

Tibor Nagy,1 Kaveh Emami,1 Carlos M. G. A. Fontes,2 Luis M. A. Ferreira,2 David R. Humphry,3 and Harry J. Gilbert1*

Department of Biological and Nutritional Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU,1 School of Sciences, University of Sunderland, Sunderland SR1 3SD, United Kingdom,3 CIISA-Faculdade de Medicina Veterinaria, 1199 Lisbon Codex, Portugal2

Received 1 April 2002/ Accepted 27 May 2002

The microbial degradation of xylan is a key biological process. Hardwood 4-O-methyl-D-glucuronoxylans are extensively decorated with 4-O-methyl-D-glucuronic acid, which is cleaved from the polysaccharides by {alpha}-glucuronidases. In this report we describe the primary structures of the {alpha}-glucuronidase from Cellvibrio mixtus (C. mixtus GlcA67A) and the {alpha}-glucuronidase from Pseudomonas cellulosa (P. cellulosa GlcA67A) and characterize P. cellulosa GlcA67A. The primary structures of C. mixtus GlcA67A and P. cellulosa GlcA67A, which are 76% identical, exhibit similarities with {alpha}-glucuronidases in glycoside hydrolase family 67. The membrane-associated pseudomonad {alpha}-glucuronidase released 4-O-methyl-D-glucuronic acid from 4-O-methyl-D-glucuronoxylooligosaccharides but not from 4-O-methyl-D-glucuronoxylan. We propose that the role of the glucuronidase, in combination with cell-associated xylanases, is to hydrolyze decorated xylooligosaccharides, generated by extracellular hemicellulases, to xylose and 4-O-methyl-D-glucuronic acid, enabling the pseudomonad to preferentially utilize the sugars derived from these polymers.

* Corresponding author. Mailing address: Department of Biological and Nutritional Sciences, University of Newcastle upon Tyne, New-castle upon Tyne NE1 7RU, United Kingdom. Phone: 44(191)2226962.Fax: 44(191)2228684. E-mail: H.J.Gilbert{at}Newcastle.ac.uk.

Journal of Bacteriology, September 2002, p. 4925-4929, Vol. 184, No. 17
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.17.4925-4929.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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