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Journal of Bacteriology, September 2002, p. 5058-5066, Vol. 184, No. 18
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.18.5058-5066.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Temperature- and H-NS-Dependent Regulation of a Plasmid-Encoded Virulence Operon Expressing Escherichia coli Hemolysin

Cristina Madrid,1 José M. Nieto,1 Sònia Paytubi,1 Maurizio Falconi,2 Claudio O. Gualerzi,2 and Antonio Juárez1*

Departament de Microbiologia, Universitat de Barcelona, 08028 Barcelona, Spain,1 Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032 Camerino, Italy2

Received 18 January 2002/ Accepted 18 June 2002

Proteins H-NS and Hha form a nucleoprotein complex that modulates expression of the thermoregulated hly operon of Escherichia coli. We have been able to identify two H-NS binding sites in the hly regulatory region. One of them partially overlaps the promoter region (site II), and the other is located about 2 kbp upstream (site I). In contrast, Hha protein did not show any preference for specific sequences. In vitro, temperature influences the affinity of H-NS for a DNA fragment containing both binding sites and H-NS-mediated repression of hly operon transcription. Deletion analysis of the hly regulatory region confirms the relevance of site I for thermoregulation of this operon. We present a model to explain the temperature-modulated repression of the hly operon, based on the experiments reported here and other, preexisting data.

* Corresponding author. Mailing address: Departament de Microbiologia, Universitat de Barcelona, Avda. Diagonal 645, 08028 Barcelona, Spain. Phone: 34 934034624. Fax: 34 934034629. E-mail: juarez{at}porthos.bio.ub.es.

Journal of Bacteriology, September 2002, p. 5058-5066, Vol. 184, No. 18
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.18.5058-5066.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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