This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ho, T. D.
Right arrow Articles by Slauch, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ho, T. D.
Right arrow Articles by Slauch, J. M.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2002, p. 5234-5239, Vol. 184, No. 19
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.19.5234-5239.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of GtgE, a Novel Virulence Factor Encoded on the Gifsy-2 Bacteriophage of Salmonella enterica Serovar Typhimurium

Theresa D. Ho,1,{dagger} Nara Figueroa-Bossi,2 Minhua Wang,1 Sergio Uzzau,2,{ddagger} Lionello Bossi,2 and James M. Slauch1,3*

Department of Microbiology,1 College of Medicine, University of Illinois, Urbana, Illinois 61801,3 Centre de Genetique, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France2

Received 23 April 2002/ Accepted 2 July 2002

The Gifsy-2 temperate bacteriophage of Salmonella enterica serovar Typhimurium contributes significantly to the pathogenicity of strains that carry it as a prophage. Previous studies have shown that Gifsy-2 encodes SodCI, a periplasmic Cu/Zn superoxide dismutase, and at least one additional virulence factor. Gifsy-2 encodes a Salmonella pathogenicity island 2 type III secreted effector protein. Sequence analysis of the Gifsy-2 genome also identifies several open reading frames with homology to those of known virulence genes. However, we found that null mutations in these genes did not individually have a significant effect on the ability of S. enterica serovar Typhimurium to establish a systemic infection in mice. Using deletion analysis, we have identified a gene, gtgE, which is necessary for the full virulence of S. enterica serovar Typhimurium Gifsy-2 lysogens. Together, GtgE and SodCI account for the contribution of Gifsy-2 to S. enterica serovar Typhimurium virulence in the murine model.

* Corresponding author. Mailing address: Department of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, MC-110, 601 S. Goodwin Ave., Urbana, IL 61801. Phone: (217) 244-1956. Fax: (217) 244-6697. E-mail: j-slauch{at}uiuc.edu.

{dagger} Present address: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.

{ddagger} Present address: Department of Biomedical Sciences University of Sassari, 07100 Sassari, Italy.

Journal of Bacteriology, October 2002, p. 5234-5239, Vol. 184, No. 19
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.19.5234-5239.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Flanigan, A., Gardner, J. F. (2007). Interaction of the Gifsy-1 Xis Protein with the Gifsy-1 attP Sequence. J. Bacteriol. 189: 6303-6311 [Abstract] [Full Text]  
  • Krishnakumar, R., Kim, B., Mollo, E. A., Imlay, J. A., Slauch, J. M. (2007). Structural Properties of Periplasmic SodCI That Correlate with Virulence in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 189: 4343-4352 [Abstract] [Full Text]  
  • Slominski, B., Calkiewicz, J., Golec, P., Wegrzyn, G., Wrobel, B. (2007). Plasmids derived from Gifsy-1/Gifsy-2, lambdoid prophages contributing to the virulence of Salmonella enterica serovar Typhimurium: implications for the evolution of replication initiation proteins of lambdoid phages and enterobacteria. Microbiology 153: 1884-1896 [Abstract] [Full Text]  
  • Klumpp, J., Fuchs, T. M. (2007). Identification of novel genes in genomic islands that contribute to Salmonella typhimurium replication in macrophages. Microbiology 153: 1207-1220 [Abstract] [Full Text]  
  • Golubeva, Y. A., Slauch, J. M. (2006). Salmonella enterica Serovar Typhimurium Periplasmic Superoxide Dismutase SodCI Is a Member of the PhoPQ Regulon and Is Induced in Macrophages. J. Bacteriol. 188: 7853-7861 [Abstract] [Full Text]  
  • Hermans, A. P. H. M., Beuling, A. M., van Hoek, A. H. A. M., Aarts, H. J. M., Abee, T., Zwietering, M. H. (2006). Distribution of prophages and SGI-1 antibiotic-resistance genes among different Salmonella enterica serovar Typhimurium isolates. Microbiology 152: 2137-2147 [Abstract] [Full Text]  
  • Alonso, A., Pucciarelli, M. G., Figueroa-Bossi, N., Garcia-del Portillo, F. (2005). Increased Excision of the Salmonella Prophage ST64B Caused by a Deficiency in Dam Methylase. J. Bacteriol. 187: 7901-7911 [Abstract] [Full Text]  
  • Bucarey, S. A., Villagra, N. A., Martinic, M. P., Trombert, A. N., Santiviago, C. A., Maulen, N. P., Youderian, P., Mora, G. C. (2005). The Salmonella enterica Serovar Typhi tsx Gene, Encoding a Nucleoside-Specific Porin, Is Essential for Prototrophic Growth in the Absence of Nucleosides. Infect. Immun. 73: 6210-6219 [Abstract] [Full Text]  
  • Geddes, K., Worley, M., Niemann, G., Heffron, F. (2005). Identification of New Secreted Effectors in Salmonella enterica Serovar Typhimurium. Infect. Immun. 73: 6260-6271 [Abstract] [Full Text]  
  • Fang, F. C., Libby, S. J., Castor, M. E., Fung, A. M. (2005). Isocitrate Lyase (AceA) Is Required for Salmonella Persistence but Not for Acute Lethal Infection in Mice. Infect. Immun. 73: 2547-2549 [Abstract] [Full Text]  
  • Frye, J. G., Porwollik, S., Blackmer, F., Cheng, P., McClelland, M. (2005). Host Gene Expression Changes and DNA Amplification during Temperate Phage Induction. J. Bacteriol. 187: 1485-1492 [Abstract] [Full Text]  
  • Zhang, L., Chaudhuri, R. R., Constantinidou, C., Hobman, J. L., Patel, M. D., Jones, A. C., Sarti, D., Roe, A. J., Vlisidou, I., Shaw, R. K., Falciani, F., Stevens, M. P., Gally, D. L., Knutton, S., Frankel, G., Penn, C. W., Pallen, M. J. (2004). Regulators Encoded in the Escherichia coli Type III Secretion System 2 Gene Cluster Influence Expression of Genes within the Locus for Enterocyte Effacement in Enterohemorrhagic E. coli O157:H7. Infect. Immun. 72: 7282-7293 [Abstract] [Full Text]  
  • Coombes, B. K., Brown, N. F., Valdez, Y., Brumell, J. H., Finlay, B. B. (2004). Expression and Secretion of Salmonella Pathogenicity Island-2 Virulence Genes in Response to Acidification Exhibit Differential Requirements of a Functional Type III Secretion Apparatus and SsaL. J. Biol. Chem. 279: 49804-49815 [Abstract] [Full Text]  
  • Brussow, H., Canchaya, C., Hardt, W.-D. (2004). Phages and the Evolution of Bacterial Pathogens: from Genomic Rearrangements to Lysogenic Conversion. Microbiol. Mol. Biol. Rev. 68: 560-602 [Abstract] [Full Text]  
  • Krishnakumar, R., Craig, M., Imlay, J. A., Slauch, J. M. (2004). Differences in Enzymatic Properties Allow SodCI but Not SodCII To Contribute to Virulence in Salmonella enterica Serovar Typhimurium Strain 14028. J. Bacteriol. 186: 5230-5238 [Abstract] [Full Text]  
  • Bacciu, D., Falchi, G., Spazziani, A., Bossi, L., Marogna, G., Leori, G. S., Rubino, S., Uzzau, S. (2004). Transposition of the Heat-Stable Toxin astA Gene into a Gifsy-2-Related Prophage of Salmonella enterica Serovar Abortusovis. J. Bacteriol. 186: 4568-4574 [Abstract] [Full Text]  
  • Ellermeier, C. D., Slauch, J. M. (2004). RtsA Coordinately Regulates DsbA and the Salmonella Pathogenicity Island 1 Type III Secretion System. J. Bacteriol. 186: 68-79 [Abstract] [Full Text]  
  • Bossi, L., Fuentes, J. A., Mora, G., Figueroa-Bossi, N. (2003). Prophage Contribution to Bacterial Population Dynamics. J. Bacteriol. 185: 6467-6471 [Abstract] [Full Text]  
  • Hacker, J., Hentschel, U., Dobrindt, U. (2003). Prokaryotic Chromosomes and Disease. Science 301: 790-793 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»