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Journal of Bacteriology, October 2002, p. 5502-5507, Vol. 184, No. 19
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.19.5502-5507.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Profiling Early Osmostress-Dependent Gene Expression in Escherichia coli Using DNA Macroarrays

Arnim Weber and Kirsten Jung*

Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, D-49069 Osnabrück, Germany

Received 28 January 2002/ Accepted 27 June 2002

DNA macroarray technology was used to monitor early transcriptional alterations of Escherichia coli in response to an osmotic upshift imposed by the addition of 0.4 M NaCl. Altered mRNA levels of 152 genes were detected; 45 genes showed increased expression while the expression of the remaining 107 genes was reduced. Northern blot analysis of several selected genes differing in their relative expression values confirmed the results obtained by the array technology.

* Corresponding author. Mailing address: Universität Osnabrück, Fachbereich Biologie/Chemie, Abteilung Mikrobiologie, D-49069 Osnabrück, Germany. Phone: 49-541-969-2276. Fax: 49-541-969-2870. E-mail: jung_k{at}biologie.uni-osnabrueck.de.

Journal of Bacteriology, October 2002, p. 5502-5507, Vol. 184, No. 19
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.19.5502-5507.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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