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Journal of Bacteriology, January 2002, p. 427-432, Vol. 184, No. 2
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.2.427-432.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Respiration-Dependent Utilization of Sugars in Yeasts: a Determinant Role for Sugar Transporters

Paola Goffrini, Iliana Ferrero, and Claudia Donnini*

Istituto di Genetica, Università degli Studi di Parma, Parma, Italy

Received 9 July 2001/ Accepted 16 October 2001

In many yeast species, including Kluyveromyces lactis, growth on certain sugars (such as galactose, raffinose, and maltose) occurs only under respiratory conditions. If respiration is blocked by inhibitors, mutation, or anaerobiosis, growth does not take place. This apparent dependence on respiration for the utilization of certain sugars has often been suspected to be associated with the mechanism of the sugar uptake step. We hypothesized that in many yeast species, the permease activities for these sugars are not sufficient to ensure the high substrate flow that is necessary for fermentative growth. By introducing additional sugar permease genes, we have obtained K. lactis strains that were capable of growing on galactose and raffinose in the absence of respiration. High dosages of both the permease and maltase genes were indeed necessary for K. lactis cells to grow on maltose in the absence of respiration. These results strongly suggest that the sugar uptake step is the major bottleneck in the fermentative assimilation of certain sugars in K. lactis and probably in many other yeasts.

* Corresponding author. Mailing address: Istituto di Genetica, Università degli Studi di Parma, Parco Area delle Scienze 11A, 43100 Parma, Italy. Phone: (39) 0521-905602. Fax: (39) 0521-905604. E-mail: claudia.donnini{at}unipr.it.

Journal of Bacteriology, January 2002, p. 427-432, Vol. 184, No. 2
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.2.427-432.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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