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Journal of Bacteriology, January 2002, p. 588-591, Vol. 184, No. 2
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.2.588-591.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Direct Quantitation of the Numbers of Individual Penicillin-Binding Proteins per Cell in Staphylococcus aureus

Michael J. Pucci* and Thomas J. Dougherty,{dagger}

Department of Microbiology, Bristol-Myers Squibb Pharmaceutical Research Institute, Wallingford, Connecticut 06492

Received 16 July 2001/ Accepted 4 October 2001

The penicillin-binding proteins (PBPs) are a set of enzymes that participate in bacterial peptidoglycan assembly. The absolute numbers of each PBP were determined by direct measurement and have been reported for two Staphylococcus aureus strains, RN4220 (methicillin-sensitive S. aureus) and RN450M (methicillin-resistant S. aureus). From the specific activity of the labeled penicillin and the absolute number of disintegrations per minute, and from the number of CFU per milliliter calculated from proteins and optical density, a determination of the number of PBPs per cell was made. These numbers ranged from approximately 150 to 825 PBPs/cell and represent the first direct determination of absolute numbers of PBPs in S. aureus.

* Corresponding author. Mailing address: Department of Microbiology (Dept. 104), Bristol-Myers Squibb Pharmaceutical Research Institute, 5 Research Pkwy., Wallingford, CT 06492. Phone: (203) 677-6372. Fax: (203) 677-6771. E-mail: pucci{at}bms.com.

{dagger} Present address: AI 2 Molecular Science, Pfizer Global Research & Development, Groton, CT 06340.

Journal of Bacteriology, January 2002, p. 588-591, Vol. 184, No. 2
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.2.588-591.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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