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Journal of Bacteriology, January 2002, p. 592-595, Vol. 184, No. 2
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.2.592-595.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Role of the RecBCD Recombination Pathway in Salmonella Virulence

David A. Cano,1 M. Graciela Pucciarelli,2 Francisco García-del Portillo,2 and Josep Casadesús1*

Departamento de Genética, Universidad de Sevilla, Seville 41080,1 Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, C.S.I.C., Campus de Cantoblanco, Madrid 28049, Spain2

Received 10 September 2001/ Accepted 24 October 2001

Mutants of Salmonella enterica lacking the RecBC function are avirulent in mice and unable to grow inside macrophages (N. A. Buchmeier, C. J. Lipps, M. Y. H. So, and F. Heffron, Mol. Microbiol. 7:933–936, 1993). The virulence-related defects of RecBC- mutants are not suppressed by sbcB and sbcCD mutations, indicating that activation of the RecF recombination pathway cannot replace the virulence-related function(s) of RecBCD. Functions of the RecF pathway such as RecJ and RecF are not required for virulence. Since the RecBCD pathway, but not the RecF pathway, is known to participate in the repair of double-strand breaks produced during DNA replication, we propose that systemic infection by S. enterica may require RecBCD-mediated recombinational repair to prime DNA replication inside phagocytes. Mutants lacking both RecD and RecJ are also attenuated in mice and are unable to proliferate in macrophages, suggesting that exonucleases V and IX provide alternative functions for RecBCD-mediated recombinational repair during Salmonella infection.

* Corresponding author. Mailing address: Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Apartado 1095, Sevilla 41080, Spain. Phone: 34 95 455 7105. Fax: 34 95 45 7104. E-mail: genbac{at}cica.es.

Journal of Bacteriology, January 2002, p. 592-595, Vol. 184, No. 2
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.2.592-595.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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