This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bergé, M.
Right arrow Articles by Martin, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bergé, M.
Right arrow Articles by Martin, B.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 2002, p. 610-613, Vol. 184, No. 2
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.2.610-613.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of a Protein That Inactivates the Competence-Stimulating Peptide of Streptococcus pneumoniae

Mathieu Bergé,1 Hanno Langen,2 Jean-Pierre Claverys,1 and Bernard Martin1*

Laboratoire de Microbiologie et Génétique Moléculaire, UMR 5100 CNRS-Université Paul Sabatier, 31062 Toulouse Cedex, France,1 Hoffmann-La Roche Ltd., CH-4070 Basel, Switzerland2

Received 16 August 2001/ Accepted 26 October 2001

Competence for genetic transformation of Streptococcus pneumoniae is a transient physiological property inducible by a competence-stimulating peptide (CSP). A 68-kDa CSP-inactivating protein was previously obtained following lithium chloride (LiCl) extraction. By the same protocol, a CSP-inactivating protein was purified and identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry as an endopeptidase, PepO. Analysis of a pepO mutant provided no support for the hypothesis that PepO participates in competence regulation. To reconcile in vitro and in vivo data, we suggest that LiCl treatment results in the release of intracellular molecules, including PepO.

* Corresponding author. Mailing address: UMR 5100 CNRS-Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex, France. Phone: (33) 561.33.59.83. Fax: (33) 561.33.58.86. E-mail: martin{at}ibcg.biotoul.fr.

Journal of Bacteriology, January 2002, p. 610-613, Vol. 184, No. 2
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.2.610-613.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cornejo, O. E, Rozen, D. E, May, R. M, Levin, B. R (2009). Oscillations in continuous culture populations of Streptococcus pneumoniae: population dynamics and the evolution of clonal suicide. Proc R Soc B 276: 999-1008 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»