This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bozonnet, S.
Right arrow Articles by Willemot, R.-M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bozonnet, S.
Right arrow Articles by Willemot, R.-M.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2002, p. 5753-5761, Vol. 184, No. 20
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.20.5753-5761.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of DSR-E, an {alpha}-1,2 Linkage-Synthesizing Dextransucrase with Two Catalytic Domains

Sophie Bozonnet, Marguerite Dols-Laffargue,{dagger} Emeline Fabre, Sandra Pizzut, Magali Remaud-Simeon, Pierre Monsan, and René-Marc Willemot*

Centre de Bioingéniérie Gilbert Durand, UMR CNRS 5504, UMR INRA 792, DGBA INSA, 31077 Toulouse Cedex 04, France

Received 22 February 2002/ Accepted 23 July 2002

A novel Leuconostoc mesenteroides NRRL B-1299 dextransucrase gene, dsrE, was isolated, sequenced, and cloned in Escherichia coli, and the recombinant enzyme was shown to be an original glucansucrase which catalyses the synthesis of {alpha}-1,6 and {alpha}-1,2 linkages. The nucleotide sequence of the dsrE gene consists of an open reading frame of 8,508 bp coding for a 2,835-amino-acid protein with a molecular mass of 313,267 Da. This is twice the average mass of the glucosyltransferases (GTFs) known so far, which is consistent with the presence of an additional catalytic domain located at the carboxy terminus of the protein and of a central glucan-binding domain, which is also significantly longer than in other glucansucrases. From sequence comparison with family 70 and {alpha}-amylase enzymes, crucial amino acids involved in the catalytic mechanism were identified, and several original sequences located at some highly conserved regions in GTFs were observed in the second catalytic domain.

* Corresponding author. Mailing address: Centre de Bioingéniérie Gilbert Durand, UMR CNRS 5504, UMR INRA 792, DGBA INSA, 135 Avenue de Rangueil, 31077 Toulouse Cedex 04, France. Phone: 33 561 559 691. Fax: 33 561 559 400. E-mail: willemot{at}insa-tlse.fr.

{dagger} Present address: Laboratoire de Biochimie et Technologie des Aliments, Talence cedex, France.

Journal of Bacteriology, October 2002, p. 5753-5761, Vol. 184, No. 20
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.20.5753-5761.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Olvera, C., Fernandez-Vazquez, J. L., Ledezma-Candanoza, L., Lopez-Munguia, A. (2007). Role of the C-terminal region of dextransucrase from Leuconostoc mesenteroides IBT-PQ in cell anchoring. Microbiology 153: 3994-4002 [Abstract] [Full Text]  
  • Moulis, C., Joucla, G., Harrison, D., Fabre, E., Potocki-Veronese, G., Monsan, P., Remaud-Simeon, M. (2006). Understanding the Polymerization Mechanism of Glycoside-Hydrolase Family 70 Glucansucrases. J. Biol. Chem. 281: 31254-31267 [Abstract] [Full Text]  
  • van Hijum, S. A. F. T., Kralj, S., Ozimek, L. K., Dijkhuizen, L., van Geel-Schutten, I. G. H. (2006). Structure-Function Relationships of Glucansucrase and Fructansucrase Enzymes from Lactic Acid Bacteria. Microbiol. Mol. Biol. Rev. 70: 157-176 [Abstract] [Full Text]  
  • Kralj, S., Stripling, E., Sanders, P., van Geel-Schutten, G. H., Dijkhuizen, L. (2005). Highly Hydrolytic Reuteransucrase from Probiotic Lactobacillus reuteri Strain ATCC 55730. Appl. Environ. Microbiol. 71: 3942-3950 [Abstract] [Full Text]  
  • Fabre, E., Bozonnet, S., Arcache, A., Willemot, R.-M., Vignon, M., Monsan, P., Remaud-Simeon, M. (2005). Role of the Two Catalytic Domains of DSR-E Dextransucrase and Their Involvement in the Formation of Highly {alpha}-1,2 Branched Dextran. J. Bacteriol. 187: 296-303 [Abstract] [Full Text]  
  • Shah, D. S. H., Joucla, G., Remaud-Simeon, M., Russell, R. R. B. (2004). Conserved Repeat Motifs and Glucan Binding by Glucansucrases of Oral Streptococci and Leuconostoc mesenteroides. J. Bacteriol. 186: 8301-8308 [Abstract] [Full Text]  
  • Kralj, S., van Geel-Schutten, G. H., Dondorff, M. M. G., Kirsanovs, S., van der Maarel, M. J. E. C., Dijkhuizen, L. (2004). Glucan synthesis in the genus Lactobacillus: isolation and characterization of glucansucrase genes, enzymes and glucan products from six different strains. Microbiology 150: 3681-3690 [Abstract] [Full Text]  
  • Kralj, S., van Geel-Schutten, G. H., van der Maarel, M. J. E. C., Dijkhuizen, L. (2004). Biochemical and molecular characterization of Lactobacillus reuteri 121 reuteransucrase. Microbiology 150: 2099-2112 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»