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Journal of Bacteriology, November 2002, p. 5966-5970, Vol. 184, No. 21
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.21.5966-5970.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Evidence for a Type III Secretion System in Aeromonas salmonicida subsp. salmonicida

Sarah E. Burr,1 Katja Stuber,1,{dagger} Thomas Wahli,2 and Joachim Frey1*

Institute of Veterinary Bacteriology,1 Centre for Fish and Wildlife Health, Institute of Animal Pathology, University of Berne, CH-3012 Berne, Switzerland2

Received 2 May 2002/ Accepted 5 August 2002

Aeromonas salmonicida subsp. salmonicida, the etiological agent of furunculosis, is an important fish pathogen. We have screened this bacterium with a broad-host-range probe directed against yscV, the gene that encodes the archetype of a highly conserved family of inner membrane proteins found in every known type III secretion system. This has led to the identification of seven open reading frames that encode homologues to proteins functioning within the type III secretion systems of Yersinia species. Six of these proteins are encoded by genes comprising a virA operon. The A. salmonicida subsp. salmonicida yscV homologue, ascV, was inactivated by marker replacement mutagenesis and used to generate an isogenic ascV mutant. Comparison of the extracellular protein profiles from the ascV mutant and the wild-type strain indicates that A. salmonicida subsp. salmonicida secretes proteins via a type III secretion system. The recently identified ADP-ribosylating toxin AexT was identified as one such protein. Finally, we have compared the toxicities of the wild-type A. salmonicida subsp. salmonicida strain and the ascV mutant against RTG-2 rainbow trout gonad cells. While infection with the wild-type strain results in significant morphological changes, including cell rounding, infection with the ascV mutant has no toxic effect, indicating that the type III secretion system we have identified plays an important role in the virulence of this pathogen.

* Corresponding author. Mailing address: Institute of Veterinary Bacteriology, University of Berne, Laenggassstrasse 122 CH-3012, Berne, Switzerland. Phone: 41 31 6312 414. Fax: 41 31 6312 634. E-mail: joachim.frey{at}vbi.unibe.ch.

{dagger} Present address: Microbiological Laboratory, Swiss Federal Veterinary Office, Bern-Liebfeld, Switzerland.

Journal of Bacteriology, November 2002, p. 5966-5970, Vol. 184, No. 21
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.21.5966-5970.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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