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Journal of Bacteriology, November 2002, p. 6056-6059, Vol. 184, No. 21
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.21.6056-6059.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Tightly Regulated Gene Expression System in Salmonella enterica Serovar Typhimurium

Jeffrey McKinney,1,2 Cecilia Guerrier-Takada,1 Jorge Galán,3 and Sidney Altman1*

Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520,1 Department of Pediatrics, Division of Infectious Diseases, Yale University, New Haven, Connecticut 06510,2 Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale School of Medicine, New Haven, Connecticut 065363

Received 25 January 2002/ Accepted 5 August 2002

A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-PBAD control element into the bacterial chromosome allows dynamic control of T7 promoter-driven RNA transcription.

* Corresponding author. Mailing address: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520. Phone: (203) 432-3500. Fax: (203) 432-5713. E-mail: sidney.altman{at}yale.edu.

Journal of Bacteriology, November 2002, p. 6056-6059, Vol. 184, No. 21
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.21.6056-6059.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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  • McKinney, J. S., Zhang, H., Kubori, T., Galan, J. E., Altman, S. (2004). Disruption of type III secretion in Salmonella enterica serovar Typhimurium by external guide sequences. Nucleic Acids Res 32: 848-854 [Abstract] [Full Text]  


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