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Journal of Bacteriology, November 2002, p. 6073-6080, Vol. 184, No. 21
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.21.6073-6080.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

A Green Nonsulfur Bacterium, Dehalococcoides ethenogenes, with the LexA Binding Sequence Found in Gram-Positive Organisms

Antonio R. Fernández de Henestrosa,1* Jordi Cuñé,1 Ivan Erill,2 Jon K. Magnuson,3 and Jordi Barbé1,4

Departament Genètica i Microbiologia,1 Centre de Recerca en Sanitat Animal, Universitat Autònoma de Barcelona,4 Biomedical Applications Group, Centro Nacional de Microelectrónica, Bellaterra, 08193 Barcelona, Spain,2 Pacific Northwest National Laboratory, Richland, Washington 993523

Received 6 May 2002/ Accepted 5 August 2002

Dehalococcoides ethenogenes is a member of the physiologically diverse division of green nonsulfur bacteria. Using a TBLASTN search, the D. ethenogenes lexA gene has been identified, cloned, and expressed and its protein has been purified. Mobility shift assays revealed that the D. ethenogenes LexA protein specifically binds to both its own promoter and that of the uvrA gene, but not to the recA promoter. Our results demonstrate that the D. ethenogenes LexA binding site is GAACN4GTTC, which is identical to that found in gram-positive bacteria. In agreement with this fact, the Bacillus subtilis DinR protein binds specifically to the D. ethenogenes LexA operator. This constitutes the first non-gram-positive bacterium exhibiting a LexA binding site identical to that of B. subtilis.

* Corresponding author. Mailing address: Universitat Autònoma de Barcelona, Departament de Genètica i Microbiologia, Unitat de Microbiologia, Edifici C, Bellaterra, 08193 Barcelona, Spain. Phone: 34 93 581 1837. Fax: 34 93 581 23 87. E-mail for Antonio R. Fernández de Henestrosa: antonio.rodriguez{at}uab.es. E-mail for Jordi Barbé jordi.barbe{at}uab.es.

Journal of Bacteriology, November 2002, p. 6073-6080, Vol. 184, No. 21
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.21.6073-6080.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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