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Journal of Bacteriology, November 2002, p. 6387-6388, Vol. 184, No. 22
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.22.6387-6388.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Protein Splicing of the Deinococcus radiodurans Strain R1 Snf2 Intein

Maurice W. Southworth and Francine B. Perler*

New England Biolabs, Beverly, Massachusetts 01915

Received 22 May 2002/ Accepted 16 August 2002

Adjacent intein fragments fused to a Snf2/Rad54 helicase-related protein and Snf2/Rad54 helicase were reported for Deinococcus radiodurans R1, leading to the speculation that a frameshift was required for splicing or that trans splicing occurred. However, a type strain (ATCC 13939, RF18410) yielded a single protein that splices by the Ala1 protein splicing pathway, with splicing dependent on adjacent residues.

* Corresponding author. Mailing address: New England Biolabs, 32 Tozer Rd., Beverly, MA 01915. Phone: (978) 927-5054. Fax: (978) 921-1350. E-mail: perler{at}neb.com.

Journal of Bacteriology, November 2002, p. 6387-6388, Vol. 184, No. 22
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.22.6387-6388.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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