This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gaballa, A.
Right arrow Articles by Helmann, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gaballa, A.
Right arrow Articles by Helmann, J. D.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2002, p. 6508-6514, Vol. 184, No. 23
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.23.6508-6514.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Functional Analysis of the Bacillus subtilis Zur Regulon

Ahmed Gaballa,1 Tao Wang,2 Rick W. Ye,2 and John D. Helmann1*

Department of Microbiology, Cornell University, Ithaca, New York 14853-8101,1 Experimental Station E328/148B, DuPont Central Research and Development, Wilmington, Delaware 198802

Received 20 June 2002/ Accepted 8 September 2002

The Bacillus subtilis zinc uptake repressor (Zur) regulates genes involved in zinc uptake. We have used DNA microarrays to identify genes that are derepressed in a zur mutant. In addition to members of the two previously identified Zur-regulated operons (yciC and ycdHI-yceA), we identified two other genes, yciA and yciB, as targets of Zur regulation. Electrophoretic mobility shift experiments demonstrated that all three operons are direct targets of Zur regulation. Zur binds to an ~28-bp operator upstream of the yciA gene, as judged by DNase I footprinting, and similar operator sites are found preceding each of the previously described target operons, yciC and ycdHI-yceA. Analysis of a yciA-lacZ fusion indicates that this operon is induced under zinc starvation conditions and derepressed in the zur mutant. Phenotypic analyses suggest that the YciA, YciB, and YciC proteins may function as part of the same Zn(II) transport pathway. Mutation of yciA or yciC, singly or in combination, had little effect on growth of the wild-type strain but significantly impaired the growth of the ycdH mutant under conditions of zinc limitation. Since the YciA, YciB, and YciC proteins are not obviously related to any known transporter family, they may define a new class of metal ion uptake system. Mutant strains lacking all three identified zinc uptake systems (yciABC, ycdHI-yceA, and zosA) are dependent on micromolar levels of added zinc for optimal growth.

* Corresponding author. Mailing address: Department of Microbiology, Wing Hall, Cornell University, Ithaca, NY 14853-8101. Phone: (607) 255-6570. Fax: (607) 255-3904. E-mail: jdh9{at}cornell.edu.

Journal of Bacteriology, December 2002, p. 6508-6514, Vol. 184, No. 23
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.23.6508-6514.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sankaran, B., Bonnett, S. A., Shah, K., Gabriel, S., Reddy, R., Schimmel, P., Rodionov, D. A., de Crecy-Lagard, V., Helmann, J. D., Iwata-Reuyl, D., Swairjo, M. A. (2009). Zinc-Independent Folate Biosynthesis: Genetic, Biochemical, and Structural Investigations Reveal New Metal Dependence for GTP Cyclohydrolase IB. J. Bacteriol. 191: 6936-6949 [Abstract] [Full Text]  
  • Gabriel, S. E., Helmann, J. D. (2009). Contributions of Zur-Controlled Ribosomal Proteins to Growth under Zinc Starvation Conditions. J. Bacteriol. 191: 6116-6122 [Abstract] [Full Text]  
  • Somerville, G. A., Proctor, R. A. (2009). At the Crossroads of Bacterial Metabolism and Virulence Factor Synthesis in Staphylococci. Microbiol. Mol. Biol. Rev. 73: 233-248 [Abstract] [Full Text]  
  • Smith, K. F., Bibb, L. A., Schmitt, M. P., Oram, D. M. (2009). Regulation and Activity of a Zinc Uptake Regulator, Zur, in Corynebacterium diphtheriae. J. Bacteriol. 191: 1595-1603 [Abstract] [Full Text]  
  • Feng, Y., Li, M., Zhang, H., Zheng, B., Han, H., Wang, C., Yan, J., Tang, J., Gao, G. F. (2008). Functional Definition and Global Regulation of Zur, a Zinc Uptake Regulator in a Streptococcus suis Serotype 2 Strain Causing Streptococcal Toxic Shock Syndrome. J. Bacteriol. 190: 7567-7578 [Abstract] [Full Text]  
  • Huang, D.-L., Tang, D.-J., Liao, Q., Li, H.-C., Chen, Q., He, Y.-Q., Feng, J.-X., Jiang, B.-L., Lu, G.-T., Chen, B., Tang, J.-L. (2008). The Zur of Xanthomonas campestris functions as a repressor and an activator of putative zinc homeostasis genes via recognizing two distinct sequences within its target promoters. Nucleic Acids Res 36: 4295-4309 [Abstract] [Full Text]  
  • Bordi, C., Butcher, B. G., Shi, Q., Hachmann, A.-B., Peters, J. E., Helmann, J. D. (2008). In Vitro Mutagenesis of Bacillus subtilis by Using a Modified Tn7 Transposon with an Outward-Facing Inducible Promoter. Appl. Environ. Microbiol. 74: 3419-3425 [Abstract] [Full Text]  
  • Gabriel, S. E., Miyagi, F., Gaballa, A., Helmann, J. D. (2008). Regulation of the Bacillus subtilis yciC Gene and Insights into the DNA-Binding Specificity of the Zinc-Sensing Metalloregulator Zur. J. Bacteriol. 190: 3482-3488 [Abstract] [Full Text]  
  • Owen, G. A., Pascoe, B., Kallifidas, D., Paget, M. S. B. (2007). Zinc-Responsive Regulation of Alternative Ribosomal Protein Genes in Streptomyces coelicolor Involves Zur and {sigma}R. J. Bacteriol. 189: 4078-4086 [Abstract] [Full Text]  
  • Shin, J.-H., Oh, S.-Y., Kim, S.-J., Roe, J.-H. (2007). The Zinc-Responsive Regulator Zur Controls a Zinc Uptake System and Some Ribosomal Proteins in Streptomyces coelicolor A3(2). J. Bacteriol. 189: 4070-4077 [Abstract] [Full Text]  
  • El Yacoubi, B., Bonnett, S., Anderson, J. N., Swairjo, M. A., Iwata-Reuyl, D., de Crecy-Lagard, V. (2006). Discovery of a New Prokaryotic Type I GTP Cyclohydrolase Family. J. Biol. Chem. 281: 37586-37593 [Abstract] [Full Text]  
  • Ollinger, J., Song, K.-B., Antelmann, H., Hecker, M., Helmann, J. D. (2006). Role of the Fur Regulon in Iron Transport in Bacillus subtilis.. J. Bacteriol. 188: 3664-3673 [Abstract] [Full Text]  
  • Akanuma, G., Nanamiya, H., Natori, Y., Nomura, N., Kawamura, F. (2006). Liberation of Zinc-Containing L31 (RpmE) from Ribosomes by Its Paralogous Gene Product, YtiA, in Bacillus subtilis.. J. Bacteriol. 188: 2715-2720 [Abstract] [Full Text]  
  • Rudolph, G., Semini, G., Hauser, F., Lindemann, A., Friberg, M., Hennecke, H., Fischer, H.-M. (2006). The Iron Control Element, Acting in Positive and Negative Control of Iron-Regulated Bradyrhizobium japonicum Genes, Is a Target for the Irr Protein. J. Bacteriol. 188: 733-744 [Abstract] [Full Text]  
  • Yuan, Z.-C., Zaheer, R., Morton, R., Finan, T. M. (2006). Genome prediction of PhoB regulated promoters in Sinorhizobium meliloti and twelve proteobacteria.. Nucleic Acids Res 34: 2686-2697 [Abstract] [Full Text]  
  • Rea, R., Hill, C., Gahan, C. G. M. (2005). Listeria monocytogenes PerR Mutants Display a Small-Colony Phenotype, Increased Sensitivity to Hydrogen Peroxide, and Significantly Reduced Murine Virulence. Appl. Environ. Microbiol. 71: 8314-8322 [Abstract] [Full Text]  
  • Jensen, S. T., Shen, L., Liu, J. S. (2005). Combining phylogenetic motif discovery and motif clustering to predict co-regulated genes. Bioinformatics 21: 3832-3839 [Abstract] [Full Text]  
  • Smits, W. K., Dubois, J.-Y. F., Bron, S., van Dijl, J. M., Kuipers, O. P. (2005). Tricksy Business: Transcriptome Analysis Reveals the Involvement of Thioredoxin A in Redox Homeostasis, Oxidative Stress, Sulfur Metabolism, and Cellular Differentiation in Bacillus subtilis. J. Bacteriol. 187: 3921-3930 [Abstract] [Full Text]  
  • Moore, C. M., Nakano, M. M., Wang, T., Ye, R. W., Helmann, J. D. (2004). Response of Bacillus subtilis to Nitric Oxide and the Nitrosating Agent Sodium Nitroprusside. J. Bacteriol. 186: 4655-4664 [Abstract] [Full Text]  
  • Diaz-Mireles, E., Wexler, M., Sawers, G., Bellini, D., Todd, J. D., Johnston, A. W. B. (2004). The Fur-like protein Mur of Rhizobium leguminosarum is a Mn2+-responsive transcriptional regulator. Microbiology 150: 1447-1456 [Abstract] [Full Text]  
  • Kindermann, B., Doring, F., Pfaffl, M., Daniel, H. (2004). Identification of Genes Responsive to Intracellular Zinc Depletion in the Human Colon Adenocarcinoma Cell Line HT-29. J. Nutr. 134: 57-62 [Abstract] [Full Text]  
  • Fuangthong, M., Helmann, J. D. (2003). Recognition of DNA by Three Ferric Uptake Regulator (Fur) Homologs in Bacillus subtilis. J. Bacteriol. 185: 6348-6357 [Abstract] [Full Text]  
  • Panina, E. M., Mironov, A. A., Gelfand, M. S. (2003). Comparative genomics of bacterial zinc regulons: Enhanced ion transport, pathogenesis, and rearrangement of ribosomal proteins. Proc. Natl. Acad. Sci. USA 100: 9912-9917 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»