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Journal of Bacteriology, February 2002, p. 672-678, Vol. 184, No. 3
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.3.672-678.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Novel Pathway for Alcoholic Fermentation of -Gluconolactone in the Yeast Saccharomyces bulderi

Johannes P. van Dijken,¹ Arjen van Tuijl,¹ Marijke A. H. Luttik,¹ Wouter J. Middelhoven,² and Jack T. Pronk^1*

Kluyver Laboratory of Biotechnology, Delft University of Technology, 2628 BC Delft,¹ Laboratorium voor Microbiologie, Wageningen University, 6700 EJ Wageningen, The Netherlands²

Received 1 July 2001/ Accepted 6 November 2001

Under anaerobic conditions, the yeast Saccharomyces bulderi rapidly ferments -gluconolactone to ethanol and carbon dioxide. We propose that a novel pathway for -gluconolactone fermentation operates in this yeast. In this pathway, -gluconolactone is first reduced to glucose via an NADPH-dependent glucose dehydrogenase (EC 1.1.1.47). After phosphorylation, half of the glucose is metabolized via the pentose phosphate pathway, yielding the NADPH required for the glucose-dehydrogenase reaction. The remaining half of the glucose is dissimilated via glycolysis. Involvement of this novel pathway in -gluconolactone fermentation in S. bulderi is supported by several experimental observations. (i) Fermentation of -gluconolactone and gluconate occurred only at low pH values, at which a substantial fraction of the substrate is present as -gluconolactone. Unlike gluconate, the latter compound is a substrate for glucose dehydrogenase. (ii) High activities of an NADP⁺-dependent glucose dehydrogenase were detected in cell extracts of anaerobic, -gluconolactone-grown cultures, but activity of this enzyme was not detected in glucose-grown cells. Gluconate kinase activity in cell extracts was negligible. (iii) During anaerobic growth on -gluconolactone, CO₂ production exceeded ethanol production by 35%, indicating that pyruvate decarboxylation was not the sole source of CO₂. (iv) Levels of the pentose phosphate pathway enzymes were 10-fold higher in -gluconolactone-grown anaerobic cultures than in glucose-grown cultures, consistent with the proposed involvement of this pathway as a primary dissimilatory route in -gluconolactone metabolism.

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* Corresponding author. Mailing address: Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands. Phone: 31 15 278 3214. Fax: 31 15 2133141. Email: j.t.pronk{at}tnw.tudelft.nl.

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Journal of Bacteriology, February 2002, p. 672-678, Vol. 184, No. 3
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.3.672-678.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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