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Journal of Bacteriology, February 2002, p. 1003-1009, Vol. 184, No. 4
0021-9193/01/$04.00+0     DOI: 10.1128/jb.184.4.1003-1009.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genetic Evidence that the uvsE Gene Product of Deinococcus radiodurans R1 Is a UV Damage Endonuclease

Ashlee M. Earl, Sara K. Rankin, Kwang-Pyo Kim, Oleana N. Lamendola, and John R. Battista*

Department of Biological Sciences, Louisiana State University and A & M College, Baton Rouge, Louisiana 70803

Received 18 September 2001/ Accepted 15 November 2001

An in vitro transposition system, developed to facilitate gene disruption in Deinococcus radiodurans R1, has been used to inactivate the gene designated dr1819 in uvrA-1+ and uvrA-1 backgrounds. dr1819 encodes a protein with homology to a UV DNA damage endonuclease expressed by Schizosaccharomyces pombe. Interruption of dr1819 greatly sensitizes the uvrA-1 strain but not the uvrA-1+ strain to UV light, indicating that the dr1819 gene product is a component in a DNA repair pathway that can compensate for the loss of nucleotide excision repair in this species. Clones of dr1819 will restore UV resistance to UVS78, a uvrA-1 uvsE strain, indicating that dr1819 and uvsE are the same locus.

* Corresponding author. Mailing address: Department of Biological Sciences, 202 Life Sciences Building, Louisiana State University, Baton Rouge, LA 70803. Phone: (225) 578-2810. Fax: (225) 578-2597. E-mail: jbattis{at}lsu.edu.

Journal of Bacteriology, February 2002, p. 1003-1009, Vol. 184, No. 4
0021-9193/01/$04.00+0     DOI: 10.1128/jb.184.4.1003-1009.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Tanaka, M., Narumi, I., Funayama, T., Kikuchi, M., Watanabe, H., Matsunaga, T., Nikaido, O., Yamamoto, K. (2005). Characterization of Pathways Dependent on the uvsE, uvrA1, or uvrA2 Gene Product for UV Resistance in Deinococcus radiodurans. J. Bacteriol. 187: 3693-3697 [Abstract] [Full Text]  
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