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Journal of Bacteriology, February 2002, p. 1196-1199, Vol. 184, No. 4
0021-9193/01/$04.00+0     DOI: 10.1128/jb.184.4.1196-1199.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

In Vitro Identification of Rns-Regulated Genes

George P. Munson, Lisa G. Holcomb, Heather L. Alexander, and June R. Scott*

Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322

Received 24 September 2001/ Accepted 30 October 2001

To identify Rns-regulated genes, a maltose binding protein (MBP)-Rns fusion protein was used to isolate DNA fragments from enterotoxigenic Escherichia coli genomic DNA that carry Rns binding sites. In vivo transcription fusion analysis shows that Rns positively regulates the expression of the open reading frame of yiiS, which lies immediately downstream of one MBP-Rns-bound fragment.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322. Phone: (404) 727-0402. Fax: (404) 727-8999. E-mail: scott{at}microbio.emory.edu.

Journal of Bacteriology, February 2002, p. 1196-1199, Vol. 184, No. 4
0021-9193/01/$04.00+0     DOI: 10.1128/jb.184.4.1196-1199.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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