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Journal of Bacteriology, February 2002, p. 1200-1203, Vol. 184, No. 4
0021-9193/01/$04.00+0     DOI: 10.1128/jb.184.4.1200-1203.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mutations in the GTPase Center of Escherichia coli 23S rRNA Indicate Release Factor 2-Interactive Sites

Wenbing Xu,,{dagger} Frances T. Pagel, and Emanuel J. Murgola*

Department of Molecular Genetics, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030

Received 15 June 2000/ Accepted 14 November 2001

Mutations in the GTPase center of Escherichia coli 23S rRNA were characterized in vivo as UGA-specific nonsense suppressors. Some site-directed mutations did not exhibit suppressor activity and were interspersed among suppressor mutations. Our results demonstrate the involvement of the two adjacent loops of this conserved rRNA structure in UGA-dependent translation termination and, taken with previous in vitro analyses and with consideration of the crystal structure of the GTPase center RNA, indicate that nucleotides 1067, 1093, 1094, and 1095 are sites of interaction with release factor 2.

* Corresponding author. Mailing address: Molecular Genetics (Box 11), M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030. Phone: (713) 792-8939. Fax: (713)-794-4295. E-mail: mannyj{at}mdanderson.org.

{dagger} Present address: P.O. Box 382009, Cambridge, MA 02238.

Journal of Bacteriology, February 2002, p. 1200-1203, Vol. 184, No. 4
0021-9193/01/$04.00+0     DOI: 10.1128/jb.184.4.1200-1203.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Scarlett, D.-J. G., McCaughan, K. K., Wilson, D. N., Tate, W. P. (2003). Mapping Functionally Important Motifs SPF and GGQ of the Decoding Release Factor RF2 to the Escherichia coli Ribosome by Hydroxyl Radical Footprinting. IMPLICATIONS FOR MACROMOLECULAR MIMICRY AND STRUCTURAL CHANGES IN RF2. J. Biol. Chem. 278: 15095-15104 [Abstract] [Full Text]  
  • Arkov, A. L., Hedenstierna, K. O. F., Murgola, E. J. (2002). Mutational Evidence for a Functional Connection between Two Domains of 23S rRNA in Translation Termination. J. Bacteriol. 184: 5052-5057 [Abstract] [Full Text]  


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