This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Noël, L.
Right arrow Articles by Bonas, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Noël, L.
Right arrow Articles by Bonas, U.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2002, p. 1340-1348, Vol. 184, No. 5
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.5.1340-1348.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Two Novel Type III-Secreted Proteins of Xanthomonas campestris pv. vesicatoria Are Encoded within the hrp Pathogenicity Island

*** Laurent Noël, Frank Thieme, Dirk Nennstiel,,{dagger} and Ulla Bonas*

Institut für Genetik, Martin-Luther-Universität Halle-Wittenberg, D-06099 Halle (Saale), Germany

Received 24 September 2001/ Accepted 4 December 2001

The Hrp type III protein secretion system (TTSS) is essential for pathogenicity of gram-negative plant pathogen Xanthomonas campestris pv. vesicatoria. cDNA-amplified fragment length polymorphism and reverse transcription-PCR analyses identified new genes, regulated by key hrp regulator HrpG, in the regions flanking the hrp gene cluster. Sequence analysis revealed genes encoding HpaG, a predicted leucine-rich repeat-containing protein, the lysozyme-like HpaH protein, and XopA and XopD, which are similar in sequence to Hpa1 from Xanthomonas oryzae pv. oryzae and PsvA from Pseudomonas syringae, respectively. XopA and XopD (Xanthomonas outer proteins) are secreted by the Xanthomonas Hrp TTSS and thus represent putative effector proteins. Mutations in xopA, but not in xopD, resulted in reduced bacterial growth in planta and delayed plant reactions in susceptible and resistant host plants. Since the xopD promoter contains a putative hrp box, which is characteristic of hrpL-regulated genes in P. syringae and Erwinia spp., the gene was probably acquired by horizontal gene transfer. Interestingly, the regions flanking the hrp gene cluster also contain insertion sequences and genes for a putative transposase and a tRNAArg. These features suggest that the hrp gene cluster of X. campestris pv. vesicatoria is part of a pathogenicity island.

* Corresponding author. Mailing address: Institut für Genetik, Martin-Luther-Universität Halle-Wittenberg, D-06099 Halle (Saale), Germany. Phone: (49) 345 5526290. Fax: (49) 345 5527277. E-mail: bonas{at}genetik.uni-halle.de.

{dagger} Present address: Bayer-AG Zentrale Forschung, D-51368 Leverkusen, Germany.

Journal of Bacteriology, March 2002, p. 1340-1348, Vol. 184, No. 5
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.5.1340-1348.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hert, A. P., Marutani, M., Momol, M. T., Roberts, P. D., Olson, S. M., Jones, J. B. (2009). Suppression of the Bacterial Spot Pathogen Xanthomonas euvesicatoria on Tomato Leaves by an Attenuated Mutant of Xanthomonas perforans. Appl. Environ. Microbiol. 75: 3323-3330 [Abstract] [Full Text]  
  • Lorenz, C., Buttner, D. (2009). Functional Characterization of the Type III Secretion ATPase HrcN from the Plant Pathogen Xanthomonas campestris pv. vesicatoria. J. Bacteriol. 191: 1414-1428 [Abstract] [Full Text]  
  • Kim, J.-G., Taylor, K. W., Hotson, A., Keegan, M., Schmelz, E. A., Mudgett, M. B. (2008). XopD SUMO Protease Affects Host Transcription, Promotes Pathogen Growth, and Delays Symptom Development in Xanthomonas-Infected Tomato Leaves. Plant Cell 20: 1915-1929 [Abstract] [Full Text]  
  • Xu, R.-Q., Blanvillain, S., Feng, J.-X., Jiang, B.-L., Li, X.-Z., Wei, H.-Y., Kroj, T., Lauber, E., Roby, D., Chen, B., He, Y.-Q., Lu, G.-T., Tang, D.-J., Vasse, J., Arlat, M., Tang, J.-L. (2008). AvrACXcc8004, a Type III Effector with a Leucine-Rich Repeat Domain from Xanthomonas campestris Pathovar campestris Confers Avirulence in Vascular Tissues of Arabidopsis thaliana Ecotype Col-0. J. Bacteriol. 190: 343-355 [Abstract] [Full Text]  
  • Oh, H.-S., Kvitko, B. H., Morello, J. E., Collmer, A. (2007). Pseudomonas syringae Lytic Transglycosylases Coregulated with the Type III Secretion System Contribute to the Translocation of Effector Proteins into Plant Cells. J. Bacteriol. 189: 8277-8289 [Abstract] [Full Text]  
  • Kvitko, B. H., Ramos, A. R., Morello, J. E., Oh, H.-S., Collmer, A. (2007). Identification of Harpins in Pseudomonas syringae pv. tomato DC3000, Which Are Functionally Similar to HrpK1 in Promoting Translocation of Type III Secretion System Effectors. J. Bacteriol. 189: 8059-8072 [Abstract] [Full Text]  
  • Oh, C.-S., Beer, S. V. (2007). AtHIPM, an Ortholog of the Apple HrpN-Interacting Protein, Is a Negative Regulator of Plant Growth and Mediates the Growth-Enhancing Effect of HrpN in Arabidopsis. Plant Physiol. 145: 426-436 [Abstract] [Full Text]  
  • Tamir-Ariel, D., Navon, N., Burdman, S. (2007). Identification of Genes in Xanthomonas campestris pv. vesicatoria Induced during Its Interaction with Tomato. J. Bacteriol. 189: 6359-6371 [Abstract] [Full Text]  
  • Koebnik, R., Kruger, A., Thieme, F., Urban, A., Bonas, U. (2006). Specific Binding of the Xanthomonas campestris pv. vesicatoria AraC-Type Transcriptional Activator HrpX to Plant-Inducible Promoter Boxes. J. Bacteriol. 188: 7652-7660 [Abstract] [Full Text]  
  • Zou, L.-f., Wang, X.-p., Xiang, Y., Zhang, B., Li, Y.-R., Xiao, Y.-l., Wang, J.-s., Walmsley, A. R., Chen, G.-y. (2006). Elucidation of the hrp Clusters of Xanthomonas oryzae pv. oryzicola That Control the Hypersensitive Response in Nonhost Tobacco and Pathogenicity in Susceptible Host Rice. Appl. Environ. Microbiol. 72: 6212-6224 [Abstract] [Full Text]  
  • Meyer, D., Cunnac, S., Gueneron, M., Declercq, C., Van Gijsegem, F., Lauber, E., Boucher, C., Arlat, M. (2006). PopF1 and PopF2, Two Proteins Secreted by the Type III Protein Secretion System of Ralstonia solanacearum, Are Translocators Belonging to the HrpF/NopX Family. J. Bacteriol. 188: 4903-4917 [Abstract] [Full Text]  
  • Thieme, F., Koebnik, R., Bekel, T., Berger, C., Boch, J., Buttner, D., Caldana, C., Gaigalat, L., Goesmann, A., Kay, S., Kirchner, O., Lanz, C., Linke, B., McHardy, A. C., Meyer, F., Mittenhuber, G., Nies, D. H., Niesbach-Klosgen, U., Patschkowski, T., Ruckert, C., Rupp, O., Schneiker, S., Schuster, S. C., Vorholter, F.-J., Weber, E., Puhler, A., Bonas, U., Bartels, D., Kaiser, O. (2005). Insights into Genome Plasticity and Pathogenicity of the Plant Pathogenic Bacterium Xanthomonas campestris pv. vesicatoria Revealed by the Complete Genome Sequence. J. Bacteriol. 187: 7254-7266 [Abstract] [Full Text]  
  • Tsuge, S., Terashima, S., Furutani, A., Ochiai, H., Oku, T., Tsuno, K., Kaku, H., Kubo, Y. (2005). Effects on Promoter Activity of Base Substitutions in the cis-Acting Regulatory Element of HrpXo Regulons in Xanthomonas oryzae pv. oryzae. J. Bacteriol. 187: 2308-2314 [Abstract] [Full Text]  
  • Weber, E., Ojanen-Reuhs, T., Huguet, E., Hause, G., Romantschuk, M., Korhonen, T. K., Bonas, U., Koebnik, R. (2005). The Type III-Dependent Hrp Pilus Is Required for Productive Interaction of Xanthomonas campestris pv. vesicatoria with Pepper Host Plants. J. Bacteriol. 187: 2458-2468 [Abstract] [Full Text]  
  • Roden, J. A., Belt, B., Ross, J. B., Tachibana, T., Vargas, J., Mudgett, M. B. (2004). A genetic screen to isolate type III effectors translocated into pepper cells during Xanthomonas infection. Proc. Natl. Acad. Sci. USA 101: 16624-16629 [Abstract] [Full Text]  
  • Kim, J.-G., Jeon, E., Oh, J., Moon, J. S., Hwang, I. (2004). Mutational Analysis of Xanthomonas Harpin HpaG Identifies a Key Functional Region That Elicits the Hypersensitive Response in Nonhost Plants. J. Bacteriol. 186: 6239-6247 [Abstract] [Full Text]  
  • Cunnac, S., Boucher, C., Genin, S. (2004). Characterization of the cis-Acting Regulatory Element Controlling HrpB-Mediated Activation of the Type III Secretion System and Effector Genes in Ralstonia solanacearum. J. Bacteriol. 186: 2309-2318 [Abstract] [Full Text]  
  • Noel, L., Thieme, F., Gabler, J., Buttner, D., Bonas, U. (2003). XopC and XopJ, Two Novel Type III Effector Proteins from Xanthomonas campestris pv. vesicatoria. J. Bacteriol. 185: 7092-7102 [Abstract] [Full Text]  
  • Kim, J.-G., Park, B. K., Yoo, C.-H., Jeon, E., Oh, J., Hwang, I. (2003). Characterization of the Xanthomonas axonopodis pv. glycines Hrp Pathogenicity Island. J. Bacteriol. 185: 3155-3166 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»