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Journal of Bacteriology, March 2002, p. 1488-1492, Vol. 184, No. 5
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.5.1488-1492.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

AmfS, an Extracellular Peptidic Morphogen in Streptomyces griseus

*** Kenji Ueda,1* Ken-Ichi Oinuma,1 Go Ikeda,1 Kuniaki Hosono,1 Yasuo Ohnishi,2 Sueharu Horinouchi,2 and Teruhiko Beppu1

Department of Applied Biological Sciences, Nihon University, Fujisawa 252-8510,1 Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan2

Received 24 September 2001/ Accepted 29 November 2001

The amf gene cluster was previously identified as a regulator for the onset of aerial-mycelium formation in Streptomyces griseus. The nucleotide sequences of amf and its counterparts in other species revealed a conserved gene organization consisting of five open reading frames. A nonsense mutation in amfS, encoding a 43-amino-acid peptide, caused significant blocking of aerial-mycelium formation and streptomycin production, suggesting its role as a regulatory molecule. Extracellular-complementation tests for the aerial-mycelium-deficient phenotype of the amfS mutant demonstrated that AmfS was secreted by the wild-type strain. A null mutation in amfBA, encoding HlyB-like membrane translocators, abolished the extracellular AmfS activity without affecting the wild-type morphology, which suggests that AmfBA is involved not in production but in export of AmfS. A synthetic C-terminal octapeptide partially induced aerial-mycelium formation in the amfS mutant, which suggests that an AmfS derivative, but not AmfS itself, serves as an extracellular morphogen.

* Corresponding author. Mailing address: Department of Applied Biological Sciences, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-8510, Japan. Phone: 81-466-84-3936. Fax: 81-466-84-3935. E-mail: ueda{at}brs.nihon-u.ac.jp.

Journal of Bacteriology, March 2002, p. 1488-1492, Vol. 184, No. 5
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.5.1488-1492.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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