This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pham, H.
Right arrow Articles by Tolmasky, M. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pham, H.
Right arrow Articles by Tolmasky, M. E.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2002, p. 1607-1616, Vol. 184, No. 6
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.6.1607-1616.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Osmoregulation of Dimer Resolution at the Plasmid pJHCMW1 mwr Locus by Escherichia coli XerCD Recombination

Huong Pham,1,2 Ken J. Dery,1,2,{dagger} David J. Sherratt,1 and Marcelo E. Tolmasky1,2*

Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom ,1 Institute of Molecular Biology and Nutrition, Department of Biological Science, College of Natural Science and Mathematics, California State University Fullerton, Fullerton, California 92834-68502

Received 21 August 2001/ Accepted 21 December 2001

Xer-mediated dimer resolution at the mwr site of plasmid pJHCMW1 is osmoregulated in Escherichia coli. Whereas under low-salt conditions, the site-specific recombination reaction is efficient, under high-salt conditions, it proceeds inefficiently. Regulation of dimer resolution is independent of H-NS and is mediated by changes in osmolarity rather than ionic effects. The low level of recombination at high salt concentrations can be overcome by high levels of PepA or by mutating the ARG box to a sequence closer to the E. coli ARG box consensus. The central region of the mwr core recombination site plays a role in regulation of site-specific recombination by the osmotic pressure of the medium.

* Corresponding author. Mailing address: Institute of Molecular Biology and Nutrition, Department of Biological Science, School of Natural Science and Mathematics, California State University Fullerton, Fullerton, CA 92834-6850. Phone: (714) 278-5263. Fax: (714) 278-3426. E-mail: mtolmasky{at}fullerton.edu.

{dagger} Present address: Department of Molecular Biology. Beckman Research Institute of the City of Hope, Duarte, CA 91010-3011.

Journal of Bacteriology, March 2002, p. 1607-1616, Vol. 184, No. 6
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.6.1607-1616.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Trigueros, S., Tran, T., Sorto, N., Newmark, J., Colloms, S. D., Sherratt, D. J., Tolmasky, M. E. (2009). mwr Xer site-specific recombination is hypersensitive to DNA supercoiling. Nucleic Acids Res 37: 3580-3587 [Abstract] [Full Text]  
  • Bui, D., Ramiscal, J., Trigueros, S., Newmark, J. S., Do, A., Sherratt, D. J., Tolmasky, M. E. (2006). Differences in Resolution of mwr-Containing Plasmid Dimers Mediated by the Klebsiella pneumoniae and Escherichia coli XerC Recombinases: Potential Implications in Dissemination of Antibiotic Resistance Genes.. J. Bacteriol. 188: 2812-2820 [Abstract] [Full Text]  
  • Sarno, R., McGillivary, G., Sherratt, D. J., Actis, L. A., Tolmasky, M. E. (2002). Complete Nucleotide Sequence of Klebsiella pneumoniae Multiresistance Plasmid pJHCMW1. Antimicrob. Agents Chemother. 46: 3422-3427 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»